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Invitrogen
c-Kit Monoclonal Antibody (K45)
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FIGURE: 1 / 14
c-Kit Antibody (MA5-12944) in ICC/IF
Immunofluorescence analysis of c-Kit/CD117 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled c-Kit/CD117 (K45) Mouse Monoclonal Antibody (Product # MA5-12944) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2817... View More
Product Details
MA5-12944
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Pig
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
K45
Immunogen
Recombinant human extracellular c-kit fragment
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10990055
Product Specific Information
MA5-12944 targets CD117 in IF/ICC and IHC (P, F) applications and shows reactivity with Human samples.
The MA5-12944 immunogen is recombinant human extracellular c-kit fragment.
Target Information
KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR
UniProt ID: (Human) P10721
Entrez Gene ID: (Human) 3815
protease binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
stem cell factor receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
cytokine binding
protein homodimerization activity
metal ion binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
MAPK cascade
activation of MAPK activity
ovarian follicle development
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
immature B cell differentiation
dendritic cell cytokine production
mast cell chemotaxis
glycosphingolipid metabolic process
inflammatory response
signal transduction
spermatogenesis
spermatid development
positive regulation of cell proliferation
germ cell migration
regulation of cell shape
visual learning
male gonad development
positive regulation of gene expression
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
stem cell population maintenance
lamellipodium assembly
hemopoiesis
T cell differentiation
erythrocyte differentiation
melanocyte differentiation
positive regulation of cell migration
positive regulation of pseudopodium assembly
actin cytoskeleton reorganization
mast cell cytokine production
somatic stem cell population maintenance
embryonic hemopoiesis
ectopic germ cell programmed cell death
hematopoietic stem cell migration
megakaryocyte development
Fc receptor signaling pathway
Kit signaling pathway
erythropoietin-mediated signaling pathway
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of programmed cell death
mast cell degranulation
positive regulation of MAPK cascade
pigmentation
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of Notch signaling pathway
positive regulation of JAK-STAT cascade
protein autophosphorylation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of developmental pigmentation
somatic stem cell division
positive regulation of long-term neuronal synaptic plasticity
digestive tract development
stem cell differentiation
epithelial cell proliferation
detection of mechanical stimulus involved in sensory perception of sound
positive regulation of sequence-specific DNA binding transcription factor activity
cell chemotaxis
mast cell differentiation
mast cell proliferation
cellular response to thyroid hormone stimulus
melanocyte migration
melanocyte adhesion
positive regulation of vascular smooth muscle cell differentiation
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