Proteintech

c-Kit/CD117 Polyclonal Antibody

c-Kit/CD117 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

c-Kit/CD117 Antibody (18696-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 18696-1-AP (c-Kit/CD117 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

c-Kit/CD117 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

c-Kit/CD117 Antibody in Western Blot (WB)

c-Kit/CD117 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

18696-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Elevated c-kit expression in failed autologous arteriovenous fistulas in end-stage renal disease patients.

The journal of vascular access

Du J,Li Y,Jia X,Kong X,Liang L,Wang D,Li A,Chen Q,Su H,Li W,Xu D

Fri Mar 01 00:00:00 UTC 2024

Elevated c-kit expression in failed autologous arteriovenous fistulas in end-stage renal disease patients.

The journal of vascular access

Du J,Li Y,Jia X,Kong X,Liang L,Wang D,Li A,Chen Q,Su H,Li W,Xu D

Fri Mar 01 00:00:00 UTC 2024

Receptor tyrosine kinase C-kit promotes a destructive phenotype of FLS in osteoarthritis via intracellular EMT signaling.

Molecular medicine (Cambridge, Mass.)

Cao X,Wu S,Wang X,Huang J,Zhang W,Liang C

Thu Mar 23 00:00:00 UTC 2023

Exposure to elevated temperature affects the expression of PIWI-interacting RNAs and associated transcripts in mouse testes.

Andrology

Chen W,Zhao Z,Cen S,Lv D,Wu J,Zhou X,Yang T,Zhao T,Hou L,Mao X

Mon May 01 00:00:00 UTC 2023

Treatment with imatinib was useful to delay the neointimal hyperplasia of aortocaval fistula in adenine-induced renal failure rats.

Biochemical and biophysical research communications

Du J,Song J,Ding L,Fan X,Lin L,Li A,Liang L,Kong X

Sun Sep 10 00:00:00 UTC 2023

Identification of the Prognostic Biomarkers CBX6 and CBX7 in Bladder Cancer.

Diagnostics (Basel, Switzerland)

Li X,Li L,Xiong X,Kuang Q,Peng M,Zhu K,Luo P

Tue Apr 11 00:00:00 UTC 2023

Acceleration of melanocyte senescence by the proinflammatory cytokines IFNγ and TNFα impairs the repigmentation response of vitiligo patients to narrowband ultraviolet B (NBUVB) phototherapy.

Mechanisms of ageing and development

Dong BQ,Liao ZK,Le Y,Jiang S,Luo LF,Miao F,Le Poole IC,Lei TC

Sat Apr 01 00:00:00 UTC 2023

Primary extra-gastrointestinal stromal tumor of retroperitoneum: Clinicopathologic characteristics and prognosis of six cases.

Frontiers in oncology

Lin J,Liao W,Wang J,Li W,Tang X,Li H,Yi X,Lu X,Chen Z,Zhu B,Feng X,Diao D

Sat Mar 11 00:00:00 UTC 2023

Preclinical Evaluation of the Multiple Tyrosine Kinases Inhibitor Anlotinib in Leukemia Stem Cells.

Pharmaceuticals (Basel, Switzerland)

Jiang Y,Liu L,Jiang Y,Li Z,Feng L,Zhuang X,Lin Z,Chen Q,Chen G,He J,Li G,Zha J,Xu B

Tue Oct 25 00:00:00 UTC 2022

Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial-Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis.

International journal of molecular sciences

Ding L,Yang Y,Lu Q,Qu D,Chandrakesan P,Feng H,Chen H,Chen X,Liao Z,Du J,Cao Z,Weygant N

Tue Nov 01 00:00:00 UTC 2022

Immune Infiltrating Cells-Derived Risk Signature Based on Large-scale Analysis Defines Immune Landscape and Predicts Immunotherapy Responses in Glioma Tumor Microenvironment.

Frontiers in immunology

Zhang N,Zhang H,Wang Z,Dai Z,Zhang X,Cheng Q,Liu Z

Mon Oct 25 00:00:00 UTC 2021

Comparative Transcriptome Analysis Reveals Relationship among mRNAs, lncRNAs, and circRNAs of Slow Transit Constipation.

BioMed research international

Yan S,Yue Y,Sun M,Chen Y,Wang X,Qian H

Tue Sep 28 00:00:00 UTC 2021

Single-Cell Sequencing Unveils the Heterogeneity of Nonimmune Cells in Chronic Apical Periodontitis.

Frontiers in cell and developmental biology

Lin X,Chi D,Meng Q,Gong Q,Tong Z

Fri Mar 04 00:00:00 UTC 2022

RNA-Seq-based transcriptomic profiling of primary interstitial cells of Cajal in response to bovine viral diarrhea virus infection.

Veterinary research communications

Li S,Hu X,Tian R,Guo Y,Chen J,Li Z,Zhao X,Kuang L,Ran D,Zhao H,Zhang X,Wang J,Xia L,Yue J,Yao G,Fu Q,Shi H

Thu Aug 01 00:00:00 UTC 2019

SDF-1α promotes repair of myocardial ischemic necrosis zones in rats.

International journal of clinical and experimental pathology

Cai X,Zhu Z,Zhang Y,Tian X

Fri Jan 17 00:00:00 UTC 2020

The role of high serum CXCL13 level in Waldenström macroglobulinemia.

Leukemia research

Wu YY,Cai H,Zhao AL,Zhang L,Zhou DB,Cao XX,Li J

Sat Jun 01 00:00:00 UTC 2019

Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.

Journal of experimental & clinical cancer research : CR

Lu W,Wan Y,Li Z,Zhu B,Yin C,Liu H,Yang S,Zhai Y,Yu Y,Wei Y,Shi J

Thu Mar 22 00:00:00 UTC 2018

Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.

Journal of experimental & clinical cancer research : CR

Lu W,Wan Y,Li Z,Zhu B,Yin C,Liu H,Yang S,Zhai Y,Yu Y,Wei Y,Shi J

Thu Mar 22 00:00:00 UTC 2018

Orai1 mediates tumor-promoting store-operated Ca²⁺ entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Wang L,Hao J,Zhang Y,Yang Z,Cao Y,Lu W,Shu Y,Jiang L,Hu Y,Lv W,Liu Y,Dong P

Wed Feb 01 00:00:00 UTC 2017

Orai1 mediates tumor-promoting store-operated Ca²⁺ entry in human gastrointestinal stromal tumors via c-KIT and the extracellular signal-regulated kinase pathway.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Wang L,Hao J,Zhang Y,Yang Z,Cao Y,Lu W,Shu Y,Jiang L,Hu Y,Lv W,Liu Y,Dong P

Wed Feb 01 00:00:00 UTC 2017

Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis.

Oncology reports

Sang XB,Sun KX,Wang LL,Chen S,Wu DD,Zong ZH,Zhao Y

Thu Dec 01 00:00:00 UTC 2016

Effects and mechanism of RhoC downregulation in suppressing ovarian cancer stem cell proliferation, drug resistance, invasion and metastasis.

Oncology reports

Sang XB,Sun KX,Wang LL,Chen S,Wu DD,Zong ZH,Zhao Y

Thu Dec 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

View more View less

Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR

View more View less

UniProt ID: (Human) P10721

View more View less

Entrez Gene ID: (Human) 3815

View more View less

Function(s)

protease binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding cytokine binding protein homodimerization activity metal ion binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

c-Kit/CD117 Polyclonal Antibody

c-Kit/CD117 Antibody (18696-1-AP) in ICC/IF

Product Details

18696-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!