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c-MAF Antibody (PA5-78051) in WB
Western Blot was performed using Anti-c-MAF Polyclonal Antibody (Product # PA5-78051) and a 42 kDa band corresponding to Transcription factor MAF was observed across the cell lines tested which decreased with differentiation to adipocytes in 3T3-L1 and increased with differentiation to Myotubules in C2C12 along with an uncharacterized band around 55 kDa(*). Nuclear enriched extracts (30 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to Adipocytes (Lane 2), C2C12 (Lane 3), C2C12 differentiated to Myotubules (Lane 4) were electroph... View More
Product Details
PA5-78051
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human c-Maf. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735540
Product Specific Information
Positive Control: HepG2
Predicted Reactivity: Mouse (100%), Rat (99%), Chicken (91%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; c-Maf long form; c-maf proto-oncogene; MGC71685; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog
Gene Aliases: 2810401A20Rik; A230108G15Rik; AW047063; AYGRP; c-MAF; CCA4; CTRCT21; MAF; Maf2
UniProt ID: (Human) O75444, (Mouse) P54843
Entrez Gene ID: (Human) 4094, (Mouse) 17132
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein homodimerization activity
protein heterodimerization activity
negative regulation of transcription from RNA polymerase II promoter
cytokine production
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
regulation of chondrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
inner ear development
lens fiber cell differentiation
lens development in camera-type eye
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of gene expression
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