Proteintech

c-Met (Cytoplasmic) Polyclonal Antibody

c-Met (Cytoplasmic) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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c-Met (Cytoplasmic) Antibody (25869-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 25869-1-AP) (c-Met (Cytoplasmic) antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

c-Met (Cytoplasmic) Antibody in Western Blot (WB)

c-Met (Cytoplasmic) Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

25869-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

c-Met (Cytoplasmic) Fusion Protein Ag23140 (965-1085 aa encoded by BC130420)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Immunogen sequence: GSELVRYDA RVHTPHLDRL VSARSVSPTT EMVSNESVDY RATFPEDQFP NSSQNGSCRQ VQYPLTDMSP ILTSGDSDIS SPLLQNTVHI DLSALNPELV QAVQHVVIGP SSLIVHFNEV IG (965-1085 aa encoded by BC130420)

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling.

International journal of molecular sciences

Xu X,Deng L,Tang Y,Li J,Zhong T,Hao X,Fan Y,Mu S

Sun Apr 30 00:00:00 UTC 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 UTC 2023

Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma.

International journal of experimental pathology

Zeng C,Wu JK,Lu X

Thu Jun 01 00:00:00 UTC 2023

Time-extended exposure of gastric epithelial cells to secretome of Helicobacter pylori-activated fibroblasts induces reprogramming of gastric epithelium towards pre-cancerogenic and pro-invasive phenotype.

American journal of cancer research

Krzysiek-Maczka G,Targosz A,Wrobel T,Paw M,Szczyrk U,Opila J,Strzalka M,Wierdak M,Major P,Brzozowski T,Czyz J,Ptak-Belowska A

Wed Apr 13 00:00:00 UTC 2022

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Cell death discovery

Song L,Zhang L,Zhou Y,Shao X,Xu Y,Pei D,Wang Q

Thu Apr 21 00:00:00 UTC 2022

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Cell death discovery

Song L,Zhang L,Zhou Y,Shao X,Xu Y,Pei D,Wang Q

Thu Apr 21 00:00:00 UTC 2022

Identification and validation of autophagy-related gene expression for predicting prognosis in patients with idiopathic pulmonary fibrosis.

Frontiers in immunology

Huang G,Xu X,Ju C,Zhong N,He J,Tang XX

Tue Oct 11 00:00:00 UTC 2022

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma.

Frontiers in oncology

Wang H,Tian RF,Liang X,Fan J,Duan ZC,Fan XY,Zhang JJ,Yao DS,Chen ZN,Li L

Thu Feb 02 00:00:00 UTC 2023

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 UTC 2022

Development of bispecific anti-c-Met/PD-1 diabodies for the treatment of solid tumors and the effect of c-Met binding affinity on efficacy.

Oncoimmunology

Yuan Q,Liang Q,Sun Z,Yuan X,Hou W,Wang Y,Wang H,Yu M

Fri Oct 15 00:00:00 UTC 2021

Combinational blockade of MET and PD-L1 improves pancreatic cancer immunotherapeutic efficacy.

Journal of experimental & clinical cancer research : CR

Li E,Huang X,Zhang G,Liang T

Fri Sep 03 00:00:00 UTC 2021

Combinational blockade of MET and PD-L1 improves pancreatic cancer immunotherapeutic efficacy.

Journal of experimental & clinical cancer research : CR

Li E,Huang X,Zhang G,Liang T

Fri Sep 03 00:00:00 UTC 2021

Hepatocyte Growth Factor Overexpression Slows the Progression of 4NQO-Induced Oral Tumorigenesis.

Frontiers in oncology

He X,Chen S,Tang Y,Zhao X,Yan L,Wu L,Wu Z,Liu W,Chen X,Wang X

Fri Apr 29 00:00:00 UTC 2022

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Molecular therapy. Nucleic acids

Cui Y,Wan H,Zhang X

Fri Mar 05 00:00:00 UTC 2021

Development and Validation of a Nomogram based on cell growth-related Biomarkers for Oral Squamous Cell Carcinoma.

Journal of Cancer

Shuai Y,Duan Y,Zhou M,Yue K,Liu D,Fang Y,Wang Y,Wu Y,Zhang Z,Wang X

Tue Aug 03 00:00:00 UTC 2021

Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.

International journal of biological sciences

Hou P,Lin T,Meng S,Shi M,Chen F,Jiang T,Li Z,Li M,Chu S,Zheng J,Bai J

Fri Mar 25 00:00:00 UTC 2022

Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

International journal of molecular sciences

Ji J,Wang Z,Sun W,Li Z,Cai H,Zhao E,Cui H

Tue Nov 09 00:00:00 UTC 2021

Combined Treatment of Cinobufotalin and Gefitinib Exhibits Potent Efficacy against Lung Cancer.

Evidence-based complementary and alternative medicine : eCAM

Han Y,Ma R,Cao G,Liu H,He L,Tang L,Li H,Luo Q

Tue Jun 15 00:00:00 UTC 2021

CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.

FEBS open bio

Zhao D,Yang Z,Chen C,Zhang Z,Yu Y,Li Z

Mon Nov 01 00:00:00 UTC 2021

Trichostatin A downregulates bromodomain and extra-terminal proteins to suppress osimertinib resistant non-small cell lung carcinoma.

Cancer cell international

Meng Y,Qian X,Zhao L,Li N,Wu S,Chen B,Sun T,Wang X

Thu Apr 15 00:00:00 UTC 2021

Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors.

Investigational new drugs

Yuan X,Sun Z,Yuan Q,Hou W,Liang Q,Wang Y,Mo W,Wang H,Yu M

Mon Feb 01 00:00:00 UTC 2021

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Clinical and translational medicine

Dong X,Luo Y,Lu S,Ma H,Zhang W,Zhu Y,Sun G,Sun X

Mon Feb 01 00:00:00 UTC 2021

Tivantinib Decreases Hepatocyte Growth Factor-Induced BCRP Expression in Hepatocellular Carcinoma HepG2 Cells.

Biological & pharmaceutical bulletin

Kobayashi K,Higai K,Mukozu T,Matsui D,Amanuma M,Yoshimine N,Ogino Y,Matsui T,Wakui N,Shinohara M,Momiyama K,Daido Y,Nagai H,Igarashi Y

Wed Jun 16 00:00:00 UTC 2021

CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.

Cell proliferation

Ying Y,Li J,Xie H,Yan H,Jin K,He L,Ma X,Wu J,Xu X,Fang J,Wang X,Zheng X,Liu B,Xie L

Sat Feb 01 00:00:00 UTC 2020

Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors.

Food & function

Xing M,Fu R,Liu Y,Wang P,Ma P,Zhu C,Fan D

Fri May 01 00:00:00 UTC 2020

Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.

The Kaohsiung journal of medical sciences

Chen JJ,Sun X,Mao QQ,Jiang XY,Zhao XG,Xu WJ,Zhong L

Sun Mar 01 00:00:00 UTC 2020

Decorin promotes apoptosis and autophagy via suppressing c-Met in HTR-8 trophoblasts.

Reproduction (Cambridge, England)

Wang Y,Zhang H,Zhang Y,Li X,Hu X,Wang X

Fri May 01 00:00:00 UTC 2020

The long noncoding RNA FTX promotes a malignant phenotype in bone marrow mesenchymal stem cells via the miR-186/c-Met axis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu L,Li X,Shi Y,Chen H

Sun Nov 01 00:00:00 UTC 2020

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Thoracic cancer

Chen Y,Tang J,Lu T,Liu F

Wed Jul 01 00:00:00 UTC 2020

Adipose-Derived Stem Cells Promote Proliferation and Invasion in Cervical Cancer by Targeting the HGF/c-MET Pathway.

Cancer management and research

Zhai Y,Wu W,Xi X,Yu R

Mon Apr 18 00:00:00 UTC 2022

Pulp stem cells with hepatocyte growth factor overexpression exhibit dual effects in rheumatoid arthritis.

Stem cell research & therapy

Dong X,Kong F,Liu C,Dai S,Zhang Y,Xiao F,Zhang H,Wu CT,Wang H

Wed Jun 10 00:00:00 UTC 2020

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Autophagy

Huang X,Gan G,Wang X,Xu T,Xie W

Mon Jul 01 00:00:00 UTC 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 UTC 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 UTC 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 UTC 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 UTC 2019

SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.

Frontiers in oncology

Liu R,Shuai Y,Luo J,Zhang Z

Thu Oct 01 00:00:00 UTC 2020

A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.

Investigational new drugs

Hou W,Yuan Q,Yuan X,Wang Y,Mo W,Wang H,Yu M

Tue Oct 01 00:00:00 UTC 2019

Rosiglitazone Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells by Promoting PPAR-γ Activation and Subsequent Regulation of TGF-β1 and HGF Expression.

Oxidative medicine and cellular longevity

Liu YD,Yu SL,Wang R,Liu JN,Jin YS,Li YF,An RH

Mon Apr 20 00:00:00 UTC 2020

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 UTC 2018

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 UTC 2018

Clinicopathologic Features and Prognostic Factors in Alpha-Fetoprotein-Producing Colorectal Cancer: Analysis of 78 Cases.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Feng Y,Li Y,Dai W,Mo S,Li Q,Cai S

Fri Jan 25 00:00:00 UTC 2019

Clinicopathologic Features and Prognostic Factors in Alpha-Fetoprotein-Producing Colorectal Cancer: Analysis of 78 Cases.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Feng Y,Li Y,Dai W,Mo S,Li Q,Cai S

Fri Jan 25 00:00:00 UTC 2019

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Molecular therapy. Nucleic acids

Cui Y,Yang X,Zhang X

Fri Dec 15 00:00:00 UTC 2017

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Molecular therapy. Nucleic acids

Cui Y,Yang X,Zhang X

Fri Dec 15 00:00:00 UTC 2017

SOCS1 regulates hepatic regenerative response and provides prognostic makers for acute obstructive cholangitis.

Scientific reports

Yu J,Zhang W,Qian H,Tang H,Lin W,Lu B

Fri Aug 25 00:00:00 UTC 2017

SOCS1 regulates hepatic regenerative response and provides prognostic makers for acute obstructive cholangitis.

Scientific reports

Yu J,Zhang W,Qian H,Tang H,Lin W,Lu B

Fri Aug 25 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: c-Met; cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

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Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2

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UniProt ID: (Dog) Q75ZY9, (Human) P08581, (Rat) P97523

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Entrez Gene ID: (Dog) 403438, (Human) 4233, (Rat) 24553, (Mouse) 17295

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Function(s)

transmembrane receptor protein tyrosine kinase activity ATP binding protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding protein phosphatase binding protein kinase activity beta-catenin binding protein complex binding phospholipase binding phosphatidylinositol 3-kinase binding protein heterodimerization activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Met (Cytoplasmic) Polyclonal Antibody

c-Met (Cytoplasmic) Antibody (25869-1-AP) in IHC (P)

Product Details

25869-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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