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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Myc Tag Antibodies

          GenScript

          c-Myc-tag Polyclonal Antibody

          16 References
          View all (104) Myc Tag antibodies
          Datasheet
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          Datasheet
          Protocols
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          Cite c-Myc-tag Polyclonal Antibody

          • Antibody Testing Data (4)
          c-Myc-tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          c-Myc-tag Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          c-Myc-tag Antibody (A00172-40) in WB

          Western blot analysis of c-Myc fusion protein (MW~52KD) using 1 µg/mL Rabbit Anti-c-Myc-tag Polyclonal Antibody (GenScript, A00172). Lane 1-3: c-Myc fusion protein in HEK293 cell lysate. Lane 4: Negative HEK293 cell lysate. Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000). The signal was developed with LumiSensor TM HRP Substrate Kit (GenScript, L00221500). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          c-Myc-tag Antibody in Western Blot (WB)
          c-Myc-tag Antibody in Western Blot (WB)
          c-Myc-tag Antibody in Western Blot (WB)
          c-Myc-tag Antibody in Western Blot (WB)

          Product Details

          A00172-40

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-1.0 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5-20 µg/mL
          -

          ELISA (ELISA)

          0.05-0.2 µg/mL
          -

          Immunoprecipitation (IP)

          2-5 µg/mL
          -

          Miscellaneous PubMed (MISC)

          -
          View 16 publications 16 publications
          Product Specifications

          Species Reactivity

          Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.

          The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

          Target Information

          Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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