Invitrogen

c-Raf Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-29333

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Raf1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2546809

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (96%), Rat (97%), Chicken (92%), Rhesus Monkey (100%), Bovine (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-Raf proto-oncogene, serine/threonine kinase; cRaf; Craf1 transforming; EC 2.7.11.1; kinase Raf1; murine sarcoma 3611 oncogene 1; Oncogene RAF1; OTTHUMP00000207813; OTTHUMP00000209389; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; sarcoma 3611 oncogene; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; vraf1 murine le

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Gene Aliases: 6430402F14Rik; AA990557; BB129353; c-Raf; CMD1NN; CRAF; Craf1; D830050J10Rik; NS5; RAF; Raf-1; RAF1; v-Raf

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UniProt ID: (Human) P04049, (Mouse) Q99N57

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Entrez Gene ID: (Human) 5894, (Mouse) 110157

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding kinase activity small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding metal ion binding protein heterodimerization activity nucleotide binding receptor signaling protein activity transferase activity transferase activity, transferring phosphorus-containing groups Ras GTPase binding
Process(es)
MAPK cascade activation of MAPKK activity response to hypoxia cellular glucose homeostasis positive regulation of protein phosphorylation protein phosphorylation signal transduction negative regulation of cell proliferation phosphorylation cell differentiation negative regulation of protein complex assembly positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance intracellular signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch negative regulation of apoptotic process intermediate filament cytoskeleton organization positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors stimulatory C-type lectin receptor signaling pathway apoptotic process activation of adenylate cyclase activity heart development cell proliferation platelet activation thyroid gland development ion transmembrane transport regulation of Rho protein signal transduction wound healing regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cell differentiation thymus development face development regulation of cell motility
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