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cAMP Antibody (A01509-100) in ELISA
cAMP Competitive ELISA. 1.ELISA plate was coated with goat anti mouse IgG antibody. 2.Anti-cAMP monoclonal antibody (Cat. No. A01509) at appropriate dilution and cAMP standards or testing compounds were added into appropriate reaction wells. 3.After a period of incubation, cAMP-HRP conjugate (Cat.No.M01059) was added followed by proper period of incubation. 4.ELISA plate was washed with ELISA washing buffer, then TMB substrate was added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.
Product Details
A01509-100
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
4H2B6
Immunogen
3', 5'-cyclic AMP-8-KLH
Conjugate
Form
Lyophilized
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Adenosine 3, 5-cyclic monophosphate (cyclic AMP; cAMP) is one of the most important intracellular secondary messengers for transduction events. cAMP is also involved in regulating neuronal, glandular, cardiovascular, immune, and other functions and actions. A number of hormones, such as ACTH, TSH, FSH, and LH, are known to activate cAMP through the action of the enzyme adenylate cyclase, which converts ATP to cAMP. There remains considerable interest in the measurement of intracellular cAMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states. Due to the involvement of cAMP in amplifying the response of ligand binding, the second messenger cAMP has been largely employed to monitor the activation of GPCR to facilitate therapeutic drug discovery.GenScript THE™ cAMP Antibody, mAb, Mouse is developed using 3, 5-cyclic AMP-8-KLH as immunogen.
Target Information
Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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