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cAMP Antibody (MA5-44556) in ELISA
Competitive ELISA detecting cAMP in HeLa cells. The 96-well plate was coated with 1 µg/mL of Goat anti-rabbit IgG (50 µl/well). 0.05 µg/mL of cAMP Recombinant Monoclonal antibody (Product # MA5-44556) (50 µl/well) was added and incubated. After wash and block, HeLa cell lysate (~105 cell/mL) or control (lysate buffer only) (25 µl/well) were added along with 25 µl/well of 1:5,000 diluted HRP conjugated cAMP. TMB was used to develop the color after incubation and wash.
Product Details
MA5-44556
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM466
Immunogen
KLH-conjugated cAMP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2926686
Product Specific Information
This antibody reacts to human IDH1(R132H)
Target Information
Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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