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Invitrogen
cGAS Recombinant Rabbit Monoclonal Antibody (10H1L5)
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cGAS Antibody (703149) in WB
Western blot analysis was performed on whole Cell extracts (30 µg lysate) of RAW 264.7 (Lane 1), RAW 264.7 transfected with poly dA:dT (1.33 µg/mL for 16 hrs) (Lane 2) and NIH/3T3 (Lane 3). The blot was probed with Anti-cGAS (Mouse) Recombinant Rabbit Monoclonal Antibody (Product # 703149, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). A 59 kDa band corresponding to cGAS (Mouse) was observed across the cell lin... View More
Product Details
703149
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H1L5
Immunogen
Protein corresponding to mouse cGAS [aa147-aa507]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809227
Product Specific Information
This antibody is predicted to react with Monkey, Rat, Pig.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2', 5' phosphodiester linkage at the GpA step and the formation of 3', 5' phosphodiester linkage at the ApG step. This produces c[G(2',5')pA(3'5')p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 2'3'-cGAMP synthase; C6orf150; cGAMP synthase; Cyclic GMP-AMP synthase; h-cGAS; Mab-21 domain-containing protein 1; MB21D1
Gene Aliases: Cgas; E330016A19Rik; Mb21d1
UniProt ID: (Mouse) Q8C6L5
Entrez Gene ID: (Mouse) 214763
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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