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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • eGFP Antibodies

          Invitrogen

          eGFP Polyclonal Antibody

          Advanced Verification
          11 Published Figures
          20 References
          View all (10) eGFP antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite eGFP Polyclonal Antibody

          • Antibody Testing Data (4)
          • Published Figures (11)
          • Advanced Verification (1)
          eGFP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          eGFP Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          eGFP Antibody (CAB4211) in ICC/IF

          Immunofluorescent analysis of GFP Tag was performed using H3-GFP construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with eGFP Polyclonal Antibody (Product # CAB4211) at 1:100 dilution and Histone H3 Monoclonal Antibody (865R2) Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          eGFP Antibody in Immunocytochemistry (ICC/IF)
          eGFP Antibody in Immunocytochemistry (ICC/IF)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Immunohistochemistry (IHC)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody in Western Blot (WB)
          eGFP Antibody

          Product Details

          CAB4211

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 11 publications 11 publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Tag

          Published species

          Tag

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Purified recombinant eGFP

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          BBS, pH 8.4

          Contains

          no preservative

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10709851

          Product Specific Information

          CAB4211 detects eGFP in samples.

          CAB4211 has been successfully used in Western Blot and ICC/IF applications.

          The CAB4211 immunogen is purified recombinant eGFP.

          Target Information

          eGFP (green fluorescent protein) fluorescent protein.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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