Invitrogen

eGFP Monoclonal Antibody (F56-6A1.2.3)

eGFP Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

eGFP Antibody (MA1-952) in ICC/IF

Immunofluorescent analysis of GFP Tag was performed using H3-GFP construct transfected in HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with eGFP Monoclonal Antibody (F56-6A1.2.3) (Product # MA1-952) at 1:100 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mous... View More

Product Image Gallery

eGFP Antibody — Antibody specificity was demonstrated by detection of different targets fused to GFP tag in transiently transfected lysates tested. Relative detection of GFP tag was observed across different proteins fused with GFP in H3-GFP (Lane 3-5) and p65-GFP (Lane 6). GFP-variant, YFP is also being detected in His-p65-YFP lysate (Lane 7), using Anti-eGFP Monoclonal Antibody (F56-6A1.2.3) (Product # MA1-952) in Western Blot. {RE}

Product Details

MA1-952

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:20-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

F56-6A1.2.3

Immunogen

Full length enhanced green fluorescent protein (eGFP)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_889471

Product Specific Information

MA1-952 contains 100 µg of in vitro produced, Protein A purified IgG (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-952 detects eGFP and eGFP tagged proteins.

MA1-952 has been successfully used in Western blot and immunofluorescence applications.

The MA1-952 immunogen is full length enhanced green fluorescent protein (eGFP).

Target Information

eGFP (green fluorescent protein) fluorescent protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heterotypic neutrophil-in-tumor structure: A novel pathological feature first discovered in the tissues of OPSCC.

Frontiers in oncology

Fan J,Li P,Fang Q,Yang Y,Zhang H,Du W,Liu S,Luo R

MA1-952 was used in Western Blotting to imply that reduce CDKN2A promoted the formation of hNiT while elevated CDKN2A impeded the hNiT formation.

Wed Sep 07 00:00:00 UTC 2022

Differential modulation of excitatory and inhibitory populations of superficial dorsal horn neurons in lumbar spinal cord by Aβ-fiber electrical stimulation.

Pain

Fan W,Sdrulla AD

MA1-952 was used in Immunohistochemistry to suggest that Aβ-ES differentially modulates lumbar spinal cord SDH populations in a cell type- and input-specific manner.

Wed Jul 01 00:00:00 UTC 2020

The Minor Matrix Protein VP24 from Ebola Virus Lacks Direct Lipid-Binding Properties.

Viruses

Su Y,Stahelin RV

MA1-952 was used in Immunocytochemistry-immunoflourescence to demonstrate that the presence of the EBOV major matrix protein VP40 did not promote VP24 membrane association in vitro or in cells.

Sat Aug 08 00:00:00 UTC 2020

The Minor Matrix Protein VP24 from Ebola Virus Lacks Direct Lipid-Binding Properties.

Viruses

Su Y,Stahelin RV

MA1-952 was used in Immunocytochemistry-immunoflourescence to demonstrate that the presence of the EBOV major matrix protein VP40 did not promote VP24 membrane association in vitro or in cells.

Sat Aug 08 00:00:00 UTC 2020

Characterization of Rare Variants in MC4R in African American and Latino Children With Severe Early-Onset Obesity.

The Journal of clinical endocrinology and metabolism

De Rosa MC,Chesi A,McCormack S,Zhou J,Weaver B,McDonald M,Christensen S,Liimatta K,Rosenbaum M,Hakonarson H,Doege CA,Grant SFA,Hirschhorn JN,Thaker VV

MA1-952 was used in Western Blotting to identify the prevalence of MC4R mutations in children with severe early-onset obesity of African American or Latino ancestry.

Mon Jul 01 00:00:00 UTC 2019

DHX15 is up-regulated in castration-resistant prostate cancer and required for androgen receptor sensitivity to low DHT concentrations.

The Prostate

Xu Y,Song Q,Pascal LE,Zhong M,Zhou Y,Zhou J,Deng FM,Huang J,Wang Z

MA1-952 was used in Immunoprecipitation to show human castration-resistant prostate cancer specimens have upregulated DHX15 expression level which also contributes to androgen receptor sensitivity to DHT.

Wed May 01 00:00:00 UTC 2019

DHX15 is up-regulated in castration-resistant prostate cancer and required for androgen receptor sensitivity to low DHT concentrations.

The Prostate

Xu Y,Song Q,Pascal LE,Zhong M,Zhou Y,Zhou J,Deng FM,Huang J,Wang Z

Wed May 01 00:00:00 UTC 2019

Complement-independent bystander injury in AQP4-IgG seropositive neuromyelitis optica produced by antibody-dependent cellular cytotoxicity.

Acta neuropathologica communications

Duan T,Smith AJ,Verkman AS

MA1-952 was used in Immunohistochemistry-immunofluorescence to establish a novel cellular pathogenesis mechanism in AQP4-IgG seropositive NMO and provide evidence that inflammatory mechanisms can cause widespread tissue damage in NMO independently of the secondary effects from astrocyte loss.

Thu Jul 11 00:00:00 UTC 2019

In Vitro Screening Platforms for Identifying Autophagy Modulators in Mammalian Cells.

Methods in molecular biology (Clifton, N.J.)

Seranova E,Ward C,Chipara M,Rosenstock TR,Sarkar S

MA1-952 was used in Western Blotting to present stringent secondary autophagy assays to characterize the autophagy modulators arising from primary screening of mammalian cells.

Thu Jun 06 00:00:00 UTC 2019

A lanthipeptide library used to identify a protein-protein interaction inhibitor.

Nature chemical biology

Yang X,Lennard KR,He C,Walker MC,Ball AT,Doigneaux C,Tavassoli A,van der Donk WA

MA1-952 was used in Western Blotting to identify an inhibitor of the protein-protein interaction critical for HIV budding from infected cells.

Sun Apr 01 00:00:00 UTC 2018

Switchable genome editing via genetic code expansion.

Scientific reports

Suzuki T,Asami M,Patel SG,Luk LYP,Tsai YH,Perry ACF

MA1-952 was used in Western Blotting to develop a heritable Cas9-mediated mammalian genome editing system that is acutely controlled by Lysine(Boc).

Tue Jul 03 00:00:00 UTC 2018

Hypothalamic stem cells control ageing speed partly through exosomal miRNAs.

Nature

Zhang Y,Kim MS,Jia B,Yan J,Zuniga-Hertz JP,Han C,Cai D

MA1-952 was used in Immunohistochemistry to demonstrate that ageing speed is substantially controlled by hypothalamic stem cells, partially through the release of exosomal miRNAs.

Thu Aug 03 00:00:00 UTC 2017

c-Myc-Dependent Cell Competition in Human Cancer Cells.

Journal of cellular biochemistry

Patel MS,Shah HS,Shrivastava N

MA1952 was used in western blot to design a c-Myc (human homolog)-dependent in vitro cell competition model of human cancer cells in Drosophila

Sat Jul 01 00:00:00 UTC 2017

Cx31.1 acts as a tumour suppressor in non-small cell lung cancer (NSCLC) cell lines through inhibition of cell proliferation and metastasis.

Journal of cellular and molecular medicine

Zhang D,Chen C,Li Y,Fu X,Xie Y,Li Y,Huang Y

MA1-952 was used in western blot to examine the role of Cx31.1 in non-small cell lung cancer

Tue May 01 00:00:00 UTC 2012

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