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Invitrogen
eIF1 Polyclonal Antibody
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FIGURE: 1 / 5
eIF1 Antibody (PA5-143916) in ICC/IF
Immunocytochemistry analysis of eIF1 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in eIF1 Polyclonal antibody (Product # PA5-143916) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
Product Details
PA5-143916
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human EIF1 recombinant protein (Position: S2-D106).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075130
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human Jurkat whole cell, human Raji whole cell, human K562 whole cell, rat pancreas tissue, mouse pancreas tissue, mouse NIH/3T3 whole cell. IHC: human placenta tissue. ICC/IF: T-47D cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A121; eIF1; eIF13; Eukaryotic translation initiation factor 1; Protein translation factor SUI1 homolog; Sui1iso1; suppressor of initiator codon mutations, related sequence 1; suppressor of initiator codon mutations-Yeast homolog related sequence 1
Gene Aliases: A121; EIF-1; EIF1; EIF1A; ISO1; SUI1; Sui1-rs1
UniProt ID: (Human) P41567, (Mouse) P48024
Entrez Gene ID: (Human) 10209, (Rat) 287703, (Mouse) 20918
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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