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eIF2 gamma Antibody (PA5-92081) in WB
Western blot analysis of eIF2 gamma in extracts of Rat brain. Samples were incubated with eIF2 gamma Polyclonal antibody (Product # PA5-92081) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92081
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_0014061)
Conjugate
Form
Liquid
Concentration
0.26 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806375
Product Specific Information
Immunogen sequence: HLAAIEIMKL KHILILQNKI DLVKESQAKE QYEQILAFVQ GTVAEGAPII PISAQLKYNI EVVCEYIVKK IPVPPRDFTS EPRLIVIRSF DVNKPGCEVD DLKGGVAGGS ILKGVLKVGQ EIEVRPGIVS KDSEGKLMCK PIFSKIVSLF AEHNDLQYAA PGGLIGVGTK IDPTLCRADR MVGQVLGAVG ALPEIFTELE ISYFLLRRLL GVRTEGDKKA AKVQKLSKNE VLMVNIGSLS TGGRVSAVKA DLGKIVLTNP VCTEVGEKIA LSRRVEKHWR LIGWGQIRRG VTIKPTVDDD; Positive Samples: Rat brain
Target Information
The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2 alpha, eIF2 beta and eIF2 gamma (eukaryotic translation initiation factor 2 alpha, beta and gamma, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2S3 (eukaryotic translation initiation factor 2, subunit 3), also known as eIF2G, is a 472 amino acid protein that belongs to the gamma subfamily of GTP-binding elongation factor proteins. Existing as a heterotrimer composed of an alpha, beta and gamma chain, eIF2S3 functions to bind ribosomal subunits and catalyze the subsequent formation of preinitiation complexes necessary for protein synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-2-gamma; eIF2-gamma; eukaryotic translation initiation factor 2 subunit 3; Eukaryotic translation initiation factor 2 subunit 3, X-linked; Eukaryotic translation initiation factor 2 subunit gamma; Eukaryotic translation initiation factor 2 subunit gamma, X-linked; PP42
Gene Aliases: Eif2g; Eif2s3; Eif2s3x; PP42
UniProt ID: (Rat) P81795
Entrez Gene ID: (Rat) 299027
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