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eIF3h Antibody (MA5-27797) in WB
Western Blot using eIF3h Monoclonal Antibody (GT2712) (Product # MA5-27797). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with eIF3H/EIF3S3 antibody [GT2712] eIF3h Monoclonal Antibody (GT2712) (Product # MA5-27797) diluted at 1:1,000.
Product Details
MA5-27797
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT2712
Immunogen
Recombinant protein encompassing a sequence within the center region of human eIF3H/EIF3S3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735172
Product Specific Information
Positive Control: A431
Predicted Reactivity: Mouse (98%), Rat (99%), Xenopus laevis (86%), Chicken (95%), Bovine (98%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
eIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-3-gamma; eIF3 p40 subunit; EIF3H; eIF3p40; Eukaryotic translation initiation factor 3 subunit 3; Eukaryotic translation initiation factor 3 subunit H; eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD); eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD); eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa; eukaryotic translation initiation factor 3, subunit H; MGC102958
Gene Aliases: eIF3-gamma; eIF3-p40; EIF3H; EIF3S3
UniProt ID: (Human) O15372
Entrez Gene ID: (Human) 8667
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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