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eIF4A1 Antibody (711505) in ICC/IF
For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous EIF4A1 using Anti- EIF4A1 Recombinant Rabbit Superclonal™ Antibody (Product # 711505, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of EIF4A1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product #... View More
Product Details
711505
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Peptide corresponding to human eIF4A1 (aa4-aa23)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2692249
Product Specific Information
This antibody is predicted to react with Mouse, Rat, Horse, Cat
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase eIF4A-1; eIF-4A-I; eIF4A-I; Eukaryotic initiation factor 4A-I; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; initiation factor eIF-4A long form
Gene Aliases: BM-010; DDX2A; EIF-4A; eIF-4A-I; Eif4; EIF4A; eIF4A-I; EIF4A1; QtsA-12280
UniProt ID: (Human) P60842, (Mouse) P60843
Entrez Gene ID: (Human) 1973, (Cynomolgus monkey) 101926349, (Mouse) 13681
RNA cap binding
double-stranded RNA binding
mRNA binding
translation initiation factor activity
ATP-dependent RNA helicase activity
helicase activity
protein binding
ATP binding
translation factor activity, RNA binding
poly(A) RNA binding
nucleotide binding
nucleic acid binding
RNA binding
hydrolase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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