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Invitrogen
eIF4A2 Polyclonal Antibody
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FIGURE: 1 / 9
eIF4A2 Antibody (PA5-79195) in ICC/IF
Immunocytochemistry analysis of eIF4A2 using anti-eIF4A2 antibody (Product # PA5-79195) . eIF4A2 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-eIF4A2 antibody (Product # PA5-79195) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAP... View More
Product Details
PA5-79195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human eIF4A2 (5-31aa SADYNREHGGPEGMDPDGVIESNWNEI).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746311
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human U251 whole cell, human SiHa whole cell, human K562 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue . IHC: mouse spleen tissue, rat intestine tissue, human mammary cancer tissue. ICC/IF: A431 cell. Flow: SiHa cell.
Target Information
eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; eIF-4A-II; eIF4A-II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; eukaryotic translation initiation factor 4A, isoform 2; N-terminally processed; protein synthesis initiation factor 4AII
Gene Aliases: 4833432N07Rik; BM-010; DDX2B; eIF-4A-II; Eif4; EIF4A; eIF4A-II; EIF4A2; EIF4F
UniProt ID: (Human) Q14240, (Mouse) P10630, (Rat) Q5RKI1
Entrez Gene ID: (Human) 1974, (Mouse) 13682, (Rat) 303831
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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