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eIF4E3 Antibody (PA5-89499) in WB
Western blot analysis of eIF4E3 in extracts of various cell lines. Samples were incubated with eIF4E3 Polyclonal antibody (Product # PA5-89499) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89499
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human EIF4E3 (NP_7754951)
Conjugate
Form
Liquid
Concentration
1.71 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805611
Product Specific Information
Immunogen sequence: MRGERRPLWE EESNAKGGVW KMKVPKDSTS TVWKELLLAT IGEQFTDCAA ADDEVIGVSV SVRDREDVVQ VWNVNASLVG EATVLEKIYE LLPHITFKAV FYKPHEEHHA FEGGRGKH; Positive Samples: Mouse lung, Rat lung
Target Information
EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4E type 3; eIF-4E3; eIF4E type 3; eukaryotic translation initiation factor 4E member 3; Eukaryotic translation initiation factor 4E type 3; eukaryotic translation initiation factor-3
Gene Aliases: 1300018P11Rik; AI451927; eIF4E-3; Eif4e3
UniProt ID: (Mouse) Q9DBB5
Entrez Gene ID: (Rat) 297481, (Mouse) 66892
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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