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Invitrogen
eIF4G3 Polyclonal Antibody
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eIF4G3 Antibody (PA5-89943) in WB
Western blot analysis of eIF4G3 in extracts of various cell lines. Samples were incubated with eIF4G3 Polyclonal antibody (Product # PA5-89943) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-89943
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_0037512)
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805857
Product Specific Information
Immunogen sequence: HKEAKIEEQE EQRKVQQLMT KEKRRPGVQR VDEGGWNTVQ GAKNSRVLDP SKFLKITKPT IDEKIQLVPK AQLGSWGKGS SGGAKASETD ALRSSASSLN RFSALQPPAP SGSTPSTPVE FDSRRTLTSR GSMGREKNDK PLPSATARPN TFMRGGSSKD LLDNQSQEEQ RREMLETVKQ LTGGVDVERN STEAERNKTR E; Positive Samples: LO2, HeLa
Target Information
The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-4-gamma 3; eIF-4-gamma II; eIF4GII; Eukaryotic translation initiation factor 4 gamma 3; RP11-190H11.1
Gene Aliases: eIF-4G 3; eIF4G 3; EIF4G3; eIF4GII
UniProt ID: (Human) O43432
Entrez Gene ID: (Human) 8672
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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