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Invitrogen
eIF5B Polyclonal Antibody
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FIGURE: 1 / 7
eIF5B Antibody (PA5-106663) in IHC (P)
Immunohistochemistry analysis of eIF5B in human colorectal cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with eIF5B polyclonal antibody (Product # PA5-106663) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-106663
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EIF5B(Accession O60841), corresponding to amino acid residues G1053-N1103.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854331
Product Specific Information
Antibody detects endogenous levels of total EIF5B.
Target Information
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Annexin V-binding protein ABP-7; DKFZp434I036; eIF 5B; eIF-5B; Eukaryotic translation initiation factor 5B; FLJ10524; Translation initiation factor IF-2; translation initiation factor IF2
Gene Aliases: A030003E17Rik; BC018347; EIF5B; IF2; KIAA0741
UniProt ID: (Human) O60841, (Rat) B2GUV7, (Mouse) Q05D44
Entrez Gene ID: (Human) 9669, (Rat) 308306, (Mouse) 226982
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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