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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • eNOS Antibodies

          Invitrogen

          eNOS Monoclonal Antibody (9D10)

          9 Published Figures
          35 References
          View all (45) eNOS antibodies

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          Datasheet
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          Cite eNOS Monoclonal Antibody (9D10)

          • Published Figures (9)
          eNOS Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          eNOS Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          eNOS Antibody (33-4600) in WB

          International journal of molecular sciences 2022 - Low-dose tacrolimus stimulates the expression of the eNOS and rescues the phosphorylation of its active domain eNOS p-Ser 1179 in the L-NAME-challenged HTR-8/SVneo cells. ( A ) Representative WB of protein levels of eNOS p-Ser 1179 and eNOS p-Thr 495 and that of the eNOS in the HTR-8/SVneo cells after 12, 24 and 48 h of treatment with low-dose tacrolimus, L-NAME or a combination of both. ( B , C ) Histogram... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot (WB)
          eNOS Antibody in Western Blot, Immunoprecipitation (WB, IP)
          eNOS Antibody in Radioimmune assays (RIA)

          Product Details

          33-4600

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 20 publications 20 publications

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          View 3 publications 3 publications

          Radioimmune Assays (RIA)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 10 publications 10 publications
          Product Specifications

          Species Reactivity

          Bovine

          Published species

          Bovine, Human, Mouse, Non-human primate, Rat, Sheep

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          9D10

          Immunogen

          Purified bovine recombinant eNOS protein

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533121

          Target Information

          Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: cNOS; constitutive NOS; e-NOS; EC-NOS; endothelial nitric oxide synthase; endothelial NOS; eNOS; nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

          View more View less

          Gene Aliases: BOS_4740

          View more View less

          Entrez Gene ID: (Bovine) 287024

          View more View less

          Function(s)
          nitric-oxide synthase activity iron ion binding calmodulin binding FMN binding heme binding flavin adenine dinucleotide binding NADP binding
          Process(es)
          arginine catabolic process nitric oxide biosynthetic process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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