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Invitrogen
eRF1 Monoclonal Antibody (3E5)
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FIGURE: 1 / 14
eRF1 Antibody (MA5-49272) in ICC/IF
Immunocytochemistry analysis of eRF1 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in eRF1 Monoclonal antibody (Product # MA5-49272) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
MA5-49272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
3E5
Immunogen
E.coli-derived human eRF1/ETF1 recombinant protein (Position: D9-K342).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3074930
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human K562 whole cell, human Raji whole cell, human HEPG2 whole cell, human HEPG2 whole cell, rat liver tissue, rat testis tissue, rat kidney tissue, rat PC-12 whole cell, mouse liver tissue, mouse testis tissue, mouse kidney tissue, mouse RAW2647 whole cell. IHC: human tonsil tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue, rat pancreas tissue. ICC/IF: A431 cell. Flow: Caco-2 cell, HEPA1-6 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eRF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; polypeptide chain release factor 1; Protein Cl1; sup45 (yeast omnipotent suppressor 45) homolog-like 1; TB3-1
Gene Aliases: AI463371; D5S1995; D6Ertd109e; ERF; ERF1; ETF1; RF1; SUP45L1; TB3-1
UniProt ID: (Human) P62495, (Mouse) Q8BWY3, (Rat) Q5U2Q7
Entrez Gene ID: (Human) 2107, (Mouse) 225363, (Rat) 307503
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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