Proteintech

golgin 97 Polyclonal Antibody

golgin 97 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

golgin 97 Antibody (12640-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12640-1-AP (golgin 97 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

golgin 97 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

12640-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Golgin 97 Fusion Protein Ag3335 (418-767 aa encoded by BC032853)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The MW of this protein is 97 kDa, and 12640-1-AP specifically recognizes the 97 kDa protein. IF result shows strong Golgi staining.

Immunogen sequence: QIVALERMR AADQTTAEQG MRQLEQENAA LKECRNEYER SLQNHQFELK KLKEEWSQRE IVSVAMAQAL EEVRKQREEF QQQAANLTAI IDEKEQNLRE KTEVLLQKEQ EILQLERGHN SALLQIHQLQ AELEALRTLK AEEAAVVAEQ EDLLRLRGPL QAEALSVNES HVTSRAMQDP VFQLPTAGRT PNGEVGAMDL TQLQKEKQDL EQQLLEKNKT IKQMQQRMLE LRKTLQKELK IRPDNELFEV REKPGPEMAN MAPSVTNNTD LTDAREINFE YLKHVVLKFM SCRESEAFHL IKAVSVLLNF SQEEENMLKE TLEYKMSWFG SKPAPKGSIR PSISNPRIPW S (418-767 aa encoded by BC032853)

Target Information

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The Golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion.

bioRxiv : the preprint server for biology

Zhang J,Kennedy A,de Melo Jorge DM,Xing L,Reid W,Bui S,Joppich J,Rose M,Ercan S,Tang Q,Tai AW,Wang Y

Fri Mar 15 00:00:00 UTC 2024

Immunofluorescence Microscopy of the Mammalian Golgi Apparatus.

Methods in molecular biology (Clifton, N.J.)

Arab M,Nayak SC,Vitali T,Lowe M

Thu Dec 15 00:00:00 UTC 2022

Immunofluorescence Microscopy of the Mammalian Golgi Apparatus.

Methods in molecular biology (Clifton, N.J.)

Arab M,Nayak SC,Vitali T,Lowe M

Thu Dec 15 00:00:00 UTC 2022

Potential mechanisms of osthole against bladder cancer cells based on network pharmacology, molecular docking, and experimental validation.

BMC complementary medicine and therapies

Jiang Y,Zhang M,Wang L,Zhang L,Ma M,Jing M,Li J,Song R,Zhang Y,Yang Z,Zhang Y,Pu Y,Qu X,Fan J

Mon Apr 17 00:00:00 UTC 2023

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.

Cell reports

Yin S,Liu L,Ball LE,Wang Y,Bedford MT,Duncan SA,Wang H,Gan W

Tue Apr 25 00:00:00 UTC 2023

Nondegradable ubiquitinated ATG9A organizes Golgi integrity and dynamics upon stresses.

Cell reports

Luo Q,Liu Q,Cheng H,Wang J,Zhao T,Zhang J,Mu C,Meng Y,Chen L,Zhou C,Lei H,Yang J,Chen G,Li Y,Pan L,Chen Q,Zhu Y

Tue Aug 16 00:00:00 UTC 2022

Nondegradable ubiquitinated ATG9A organizes Golgi integrity and dynamics upon stresses.

Cell reports

Luo Q,Liu Q,Cheng H,Wang J,Zhao T,Zhang J,Mu C,Meng Y,Chen L,Zhou C,Lei H,Yang J,Chen G,Li Y,Pan L,Chen Q,Zhu Y

Tue Aug 16 00:00:00 UTC 2022

Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.

Nature communications

Barbier-Torres L,Murray B,Yang JW,Wang J,Matsuda M,Robinson A,Binek A,Fan W,Fernández-Ramos D,Lopitz-Otsoa F,Luque-Urbano M,Millet O,Mavila N,Peng H,Ramani K,Gottlieb R,Sun Z,Liangpunsakul S,Seki E,Van Eyk JE,Mato JM,Lu SC

Fri Jan 28 00:00:00 UTC 2022

GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.

The Journal of biological chemistry

Ahat E,Bui S,Zhang J,da Veiga Leprevost F,Sharkey L,Reid W,Nesvizhskii AI,Paulson HL,Wang Y

Mon Aug 01 00:00:00 UTC 2022

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis.

Redox biology

You M,Wu F,Gao M,Chen M,Zeng S,Zhang Y,Zhao W,Li D,Wei L,Ruan XZ,Chen Y

Tue Nov 01 00:00:00 UTC 2022

The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic.

Molecular biology of the cell

Turn RE,Hu Y,Dewees SI,Devi N,East MP,Hardin KR,Khatib T,Linnert J,Wolfrum U,Lim MJ,Casanova JE,Caspary T,Kahn RA

Tue Feb 01 00:00:00 UTC 2022

Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6.

Cell chemical biology

Qiu N,Abegg D,Guidi M,Gilmore K,Seeberger PH,Adibekian A

Thu Mar 17 00:00:00 UTC 2022

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Molecular metabolism

Zeng H,Qin H,Liao M,Zheng E,Luo X,Xiao A,Li Y,Chen L,Wei L,Zhao L,Ruan XZ,Yang P,Chen Y

Tue Mar 01 00:00:00 UTC 2022

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Molecular metabolism

Zeng H,Qin H,Liao M,Zheng E,Luo X,Xiao A,Li Y,Chen L,Wei L,Zhao L,Ruan XZ,Yang P,Chen Y

Tue Mar 01 00:00:00 UTC 2022

Endocytic recycling as cellular trafficking fate of simvastatin-loaded discoidal reconstituted high-density lipoprotein to coordinate cholesterol efflux and drug influx.

Nanomedicine : nanotechnology, biology, and medicine

Qi Z,Jiang C,Gao H,Wang Y,Zhang Q,Zhang W,Liu J

Mon Feb 01 00:00:00 UTC 2021

Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.

The Journal of biological chemistry

Merriman C,Fu D

Fri Nov 08 00:00:00 UTC 2019

GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.

Developmental cell

Zhang X,Wang L,Lak B,Li J,Jokitalo E,Wang Y

Mon Apr 23 00:00:00 UTC 2018

GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.

Developmental cell

Zhang X,Wang L,Lak B,Li J,Jokitalo E,Wang Y

Mon Apr 23 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: gap junction protein, alpha 4, 37kD; golgi autoantigen, golgin subfamily a, 1; Golgin subfamily A member 1; Golgin-97

View more View less

Gene Aliases: 0710001G09Rik; 2210418B03Rik; AW107649; GOLGA1; Golgi97; golgin-97

View more View less

UniProt ID: (Human) Q92805, (Mouse) Q9CW79

View more View less

Entrez Gene ID: (Human) 2800, (Mouse) 76899

View more View less

Function(s)

protein binding

Process(es)

protein targeting to Golgi biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

golgin 97 Polyclonal Antibody

golgin 97 Antibody (12640-1-AP) in ICC/IF

Product Details

12640-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!