Proteintech

hD53/TPD52L1 Polyclonal Antibody

hD53/TPD52L1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

hD53/TPD52L1 Antibody (14732-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

hD53/TPD52L1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

hD53/TPD52L1 Antibody in Western Blot (WB)

hD53/TPD52L1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14732-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

hD53; TPD52L1 Fusion Protein Ag6493 (1-144 aa encoded by BC002375)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.

Immunogen sequence: MEAQAQGLL ETEPLQGTDE DAVASADFSS MLSEEEKEEL KAELVQLEDE ITTLRQVLSA KERHLVEIKQ KLGMNLMNEL KQNFSKSWHD MQTTTAYKKT HETLSHAGQK ATAAFSNVGT AISKKFGDMS YSIRHSISMP AMRRK (1-144 aa encoded by BC002375)

Target Information

This gene encodes a member of the tumor protein D52 family. The encoded protein contains a coiled-coil domain and may form homo- or hetero-dimer with TPD52 family members. The protein is reported to be involved in cell proliferation and calcium signaling. It also interacts with the mitogen-activated protein kinase kinase kinase 5 and positively regulates MAP3K5-induced apoptosis. Multiple alternatively spliced transcript variants have been observed, but the full-length nature of some variants has not yet been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer.

Frontiers in oncology

Hong Q,Li B,Cai X,Lv Z,Cai S,Zhong Y,Wen B

Tue Aug 31 00:00:00 UTC 2021

Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.

Cell & bioscience

Abe Y,Mukudai Y,Kurihara M,Houri A,Chikuda J,Yaso A,Kato K,Shimane T,Shirota T

Sat Jul 03 00:00:00 UTC 2021

The Signaling Pathways Project, an integrated 'omics knowledgebase for mammalian cellular signaling pathways.

Scientific data

Ochsner SA,Abraham D,Martin K,Ding W,McOwiti A,Kankanamge W,Wang Z,Andreano K,Hamilton RA,Chen Y,Hamilton A,Gantner ML,Dehart M,Qu S,Hilsenbeck SG,Becnel LB,Bridges D,Ma'ayan A,Huss JM,Stossi F,Foulds CE,Kralli A,McDonnell DP,McKenna NJ

Thu Oct 31 00:00:00 UTC 2019

Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability.

The Biochemical journal

Motohashi H,Mukudai Y,Ito C,Kato K,Shimane T,Kondo S,Shirota T

Thu May 04 00:00:00 UTC 2017

Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.