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Invitrogen
hnRNP F Polyclonal Antibody
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FIGURE: 1 / 11
hnRNP F Antibody (PA5-79382) in ICC/IF
Immunocytochemistry analysis of HnRNPF using anti-HnRNPF antibody (Product # PA5-79382) . HnRNPF was detected in a section of SKOV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-HnRNPF antibody (Product # PA5-79382) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with D... View More
Product Details
PA5-79382
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F (23-53aa SVEDVQNFLSDCTIHDGAAGVHFIYTREGRQ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746498
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SKOV3 whole cell, human 293T whole cell, human Hela whole cell, human PANC-1 whole cell, human RT4 whole cell, human K562 whole cell, human U20S whole cell, human PC-3 whole cell, rat liver tissue, rat RH35 whole cell, rat C6 whole cell, mouse liver tissue, mouse SP2/0 whole cell, mouse HEPA1-6 whole cell. IHC: human mammary cancer tissue, human intestinal cancer tissue, mouse intestine tissue, rat intestine tissue. ICC/IF: SKOV-3 cell. Flow: U87 cell.
Target Information
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Heterogeneous nuclear ribonucleoprotein F; hnRNP F; HnRNP F protein; hnRNP H; HNRNPH1; mcs94 1; Nucleolin-like protein mcs94-1; ribonucleoprotein F; ROF
Gene Aliases: 4833420I20Rik; AA407306; HNRNPF; HNRPF; mcs94-1; OK/SW-cl.23
UniProt ID: (Human) P52597, (Rat) Q794E4, (Mouse) Q9Z2X1
Entrez Gene ID: (Human) 3185, (Rat) 64200, (Mouse) 98758
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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