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hnRNP H1 Antibody (PA5-50678) in ICC/IF
Immunofluorescence analysis of HNRNPH1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with hnRNP H1 Polyclonal Antibody (Product # PA5-50678) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at ... View More
Product Details
PA5-50678
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues near the C terminal of human heterogeneous nuclear ribonucleoprotein H1 (H)
Conjugate
Form
Liquid
Concentration
2.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2636130
Product Specific Information
The antibody detects endogenous levels of total hnRNP H1 protein.
Target Information
This gene encodes a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. This gene may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Heterogeneous nuclear ribonucleoprotein H; hnRNP H; Ratsg1
Gene Aliases: AI642080; E430005G16Rik; Hnrnph; HNRNPH1; HNRPH; HNRPH1
UniProt ID: (Human) P31943, (Mouse) O35737, (Rat) Q8VHV7
Entrez Gene ID: (Human) 3187, (Mouse) 59013, (Rat) 140931
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