Invitrogen

hnRNP K Polyclonal Antibody

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2 Published Figures
1 Reference
View all (25) hnRNP K antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-27522

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

RNA Immunoprecipitation (RIP)

1.25 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP K. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2544998

Product Specific Information

Recommended positive controls: 293T, A431, Hela, HepG2, Mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (81%), Dog (98%), Pig (99%), Rabbit (99%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progression. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CSBP; dC stretch-binding protein; dC-stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; RP11-575L7.1; Transformation up-regulated nuclear protein; transformation upregulated nuclear protein; TUNP

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Gene Aliases: AUKS; CSBP; HNRNPK; HNRPK; KBBP; NOVA; TUNP

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UniProt ID: (Human) P61978, (Rat) P61980, (Mouse) P61979

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Entrez Gene ID: (Human) 3190, (Rat) 117282, (Mouse) 15387

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding single-stranded DNA binding RNA binding mRNA 3'-UTR binding protein binding lamin binding transcription factor binding protein domain specific binding pre-mRNA 3'-splice site binding heat shock protein binding actinin binding ribonucleoprotein complex binding poly(A) RNA binding ATPase binding cadherin binding involved in cell-cell adhesion mRNA CDS binding C-rich single-stranded DNA binding core promoter proximal region DNA binding nucleic acid binding DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter mRNA splicing, via spliceosome ovarian follicle development kidney development liver development transcription from RNA polymerase II promoter RNA processing acute-phase response signal transduction peripheral nervous system development aging gene expression regulation of low-density lipoprotein particle clearance response to activity viral process protein sumoylation cerebellum development hippocampus development cerebral cortex development lung development negative regulation of protein binding cellular response to insulin stimulus positive regulation of RNA splicing camera-type eye development negative regulation of apoptotic process positive regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of transcription from RNA polymerase II promoter negative regulation of mRNA splicing, via spliceosome positive regulation of receptor-mediated endocytosis thymus development positive regulation of synaptic transmission positive regulation of dendritic spine development cellular response to amino acid stimulus cellular response to glucose stimulus regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter cellular response to rapamycin positive regulation of synapse maturation cell-cell adhesion positive regulation of long-term synaptic potentiation response to salt regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of dendrite extension cellular response to forskolin positive regulation of protein localization to cell surface negative regulation of branching morphogenesis of a nerve transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing RNA splicing regulation of transcription from RNA polymerase II promoter positive regulation of neuron projection development cellular response to organic cyclic compound
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