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Invitrogen
hnRNP L Polyclonal Antibody
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hnRNP L Antibody (PA5-79383) in ICC/IF
Immunocytochemistry analysis of HnRNP L using anti-HnRNP L antibody (Product # PA5-79383) . HnRNP L was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-HnRNP L antibody (Product # PA5-79383) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with... View More
Product Details
PA5-79383
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HnRNP L (546-571aa LLEWESKSDALETLGFLNHYQMKNPN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746499
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, human MCF-7 whole cell, human PC-3 whole cell, human U87 whole cell, rat liver tissue, mouse liver tissue, mouse RAW2647 whole cell, mouse NIH/3T3 whole cell. IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human laryngeal squamous cell carcinoma tissue, human liver cancer tissue, human lung adenocarcinoma tissue, mouse liver tissue, rat liver tissue. ICC/IF: MCF-7 cell.
Target Information
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ35509; Heterogeneous nuclear ribonucleoprotein L; heterogenous nuclear ribonucleoprotein L; hnRNP L
Gene Aliases: C79783; D830027H13Rik; Fblim1; hnRNP-L; HNRNPL; HNRPL; P/OKcl.14
UniProt ID: (Human) P14866, (Mouse) Q8R081, (Rat) F1LQ48
Entrez Gene ID: (Human) 3191, (Mouse) 15388, (Rat) 80846
mRNA processing
cellular response to amino acid starvation
positive regulation of translation
negative regulation of mRNA splicing, via spliceosome
positive regulation of mRNA binding
regulation of alternative mRNA splicing, via spliceosome
mRNA splicing, via spliceosome
RNA processing
gene expression
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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