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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • iNOS Antibodies

          Proteintech

          iNOS Polyclonal Antibody

          562 References
          View all (65) iNOS antibodies
          Datasheet
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          Datasheet
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          Cite iNOS Polyclonal Antibody

          • Antibody Testing Data (12)
          iNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          iNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          iNOS Antibody (18985-1-AP) in ICC/IF

          Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using iNOS antibody (Product # 18985-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          iNOS Antibody in Immunocytochemistry (ICC/IF)
          iNOS Antibody in Immunocytochemistry (ICC/IF)
          iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          iNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          iNOS Antibody in Western Blot (WB)
          iNOS Antibody in Western Blot (WB)
          iNOS Antibody in Western Blot (WB)
          iNOS Antibody in Flow Cytometry (Flow)

          Product Details

          18985-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:600
          View 354 publications 354 publications

          Immunohistochemistry (IHC)

          -
          View 64 publications 64 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:400
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 132 publications 132 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 1 publication 1 publication

          ELISA (ELISA)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications

          Not applicable (N/A)

          -
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Chicken, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Peptide

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.27 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody is specific to NOS2.

          Target Information

          iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: HEP-NOS; Hepatocyte NOS; hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS type II; NOS, type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

          View more View less

          Gene Aliases: HEP-NOS; i-NOS; INOS; Inosl; NOS; Nos-2; NOS-II; NOS2; NOS2A

          View more View less

          UniProt ID: (Human) P35228, (Mouse) P29477, (Rat) Q06518

          View more View less

          Entrez Gene ID: (Human) 4843, (Mouse) 18126, (Rat) 24599

          View more View less

          Function(s)
          nitric-oxide synthase activity receptor binding protein binding calmodulin binding FMN binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity metal ion binding flavin adenine dinucleotide binding NADP binding actin binding NADPH-hemoprotein reductase activity iron ion binding beta-catenin binding cAMP-dependent protein kinase regulator activity oxidoreductase activity protein kinase binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding amino acid binding
          Process(es)
          response to hypoxia positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process nitric oxide mediated signal transduction circadian rhythm response to bacterium negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity prostaglandin secretion cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration cell redox homeostasis negative regulation of blood pressure positive regulation of vasodilation regulation of insulin secretion defense response to Gram-negative bacterium positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma interleukin-6 secretion interleukin-8 secretion regulation of cytokine production involved in inflammatory response ovulation from ovarian follicle inflammatory response response to lipopolysaccharide regulation of protein kinase activity endothelial cell proliferation blood vessel remodeling signal transduction G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger aging regulation of heart contraction regulation of blood pressure response to mechanical stimulus response to hormone response to activity glycoside metabolic process cGMP-mediated signaling response to estradiol response to tumor necrosis factor intracellular signal transduction response to immobilization stress wound healing positive regulation of apoptotic process retinoic acid receptor signaling pathway cellular response to lithium ion cellular response to vitamin D cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to redox state response to dexamethasone
          It has to be done as per old AB suggested Products section.
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