Invitrogen

iNOS Recombinant Rabbit Monoclonal Antibody (HL1213)

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Datasheet
Protocols
Questions & Answers

Product Details

MA5-46960

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:2,500
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HL1213

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse iNOS. The exact sequence is proprietary.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2938032

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Target Information

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: hepatocytes; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; nitric oxide synthase-inducible; NOS type II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

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Gene Aliases: i-NOS; iNOS; Inosl; Nos-2; NOS-II; Nos2; Nos2a

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UniProt ID: (Mouse) P29477, (Rat) Q06518

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Entrez Gene ID: (Mouse) 18126, (Rat) 24599

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Function(s)
actin binding NADPH-hemoprotein reductase activity nitric-oxide synthase activity receptor binding iron ion binding protein binding calmodulin binding beta-catenin binding cAMP-dependent protein kinase regulator activity FMN binding oxidoreductase activity protein kinase binding heme binding tetrahydrobiopterin binding arginine binding protein homodimerization activity cadherin binding metal ion binding flavin adenine dinucleotide binding nitric-oxide synthase binding Hsp90 protein binding amino acid binding NADP binding
Process(es)
ovulation from ovarian follicle response to hypoxia arginine catabolic process superoxide metabolic process nitric oxide biosynthetic process inflammatory response nitric oxide mediated signal transduction negative regulation of gene expression peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to lipopolysaccharide cellular response to drug regulation of cell proliferation negative regulation of protein catabolic process defense response to bacterium negative regulation of blood pressure regulation of protein kinase activity positive regulation of vasodilation regulation of insulin secretion positive regulation of killing of cells of other organism oxidation-reduction process cellular response to lipopolysaccharide cellular response to interferon-gamma endothelial cell proliferation blood vessel remodeling signal transduction G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger aging regulation of heart contraction regulation of blood pressure response to mechanical stimulus response to hormone response to activity glycoside metabolic process cGMP-mediated signaling response to estradiol response to tumor necrosis factor intracellular signal transduction response to immobilization stress wound healing positive regulation of apoptotic process retinoic acid receptor signaling pathway defense response to Gram-negative bacterium cellular response to lithium ion cellular response to vitamin D cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to redox state response to dexamethasone
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