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Invitrogen
kaiC Polyclonal Antibody
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kaiC Antibody (PA5-144442) in WB
Western blot analysis of kaiC in recombinant protein using a dilution of 1:2,000. Samples were incubated with kaiC polyclonal antibody (Product # PA5-144442). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 56.8 kDa. Observed band size: 69 kDa.
Product Details
PA5-144442
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Nostoc sp. (strain PCC 7120/SAG 25.82/UTEX 2576) Circadian clock protein kinase KaiC (1-270aa)
Conjugate
Form
Liquid
Concentration
3.69 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3090383
Target Information
Central component of the KaiABC oscillator complex, which constitutes the main circadian regulator in cyanobacteria. Complex composition changes during the circadian cycle to control KaiC phosphorylation. KaiA stimulates KaiC autophosphorylation, while KaiB sequesters KaiA, leading to KaiC autodephosphorylation. Clock output pathways impact the RpaA transcriptional regulator. KaiC enhances the autophosphorylation activity of SasA, which then transfers its phosphate group to RpaA to activate it. KaiB and KaiC together enhance the phospho-RpaA dephosphatase activity of CikA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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