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Rockland
mCherry Polyclonal Antibody
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FIGURE: 1 / 3
mCherry Antibody (600-401-P16) in WB
Western Blot of Rabbit Anti-mCherry Antibody MX Hu Ms Rt. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: RFP (p/n 000-001-379)/HeLa WCL (p/n W09-000-364) [0.02µg/10µg]. Lane 3: HeLa WCL (p/n W09-000-364) [10µg]. Lane 4: RFP (p/n 000-001-379)/NIH/3T3 WCL (p/n W10-000-358) [0.02µg/10µg]. Lane 5: NIH/3T3 WCL (p/n W10-000-358) [10µg]. Lane 6: RFP (p/n 000-001-379)/PC-12 WCL (p/n W12-001-GL9) [0.02µg/10µg]. Lane 7: PC-12 WCL (p/n W12-001-GL9) [10µg]. Primary Antibody: Anti-mCherry at 1:1000 overnight at 2-8°C. ... View More
Product Details
600-401-P16
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
The immunogen is a mCherry mutant variant fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Target Information
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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