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OriGene
mCherry Monoclonal Antibody (OTI10G6 (formerly 10G6)), TrueMAB™
FIGURE: 1 / 1
mCherry Antibody (CF180028) in WB
Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mCherry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and blotted with anti-mCherry (TA180028, 1:2000).
Product Details
CF180028
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI10G6 (formerly 10G6)
Immunogen
This antibody is raised against a recombinant protein of mCherry expressed in HEK293 cells.
Conjugate
Form
Lyophilized
Purification
Protein A/G
Storage buffer
PBS with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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