Proteintech

mDia1 Polyclonal Antibody

View all (18) DIAPH1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (8)

mDia1 Antibody in Immunocytochemistry (ICC/IF)

mDia1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

mDia1 Antibody (20624-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

mDia1 Antibody in Immunocytochemistry (ICC/IF)

mDia1 Antibody in Immunocytochemistry (ICC/IF)

mDia1 Antibody in Western Blot (WB)

mDia1 Antibody in Western Blot (WB)

mDia1 Antibody in Western Blot (WB)

mDia1 Antibody in Western Blot (WB)

mDia1 Antibody in Western Blot (WB)

mDia1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

mDia1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

mDia1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using DIAPH1 antibody 20624-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

mDia1 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

mDia1 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

mDia1 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

mDia1 Antibody in Western Blot (WB) — COS-7 cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

mDia1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 20624-1-AP (mDia1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

mDia1 Antibody in Immunoprecipitation (IP) — IP result of anti-mDia1 (IP:20624-1-AP, 4ug; Detection:20624-1-AP 1:500) with HeLa cells lysate 1200ug.

Product Details

20624-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 6 publications 6 publications

Immunoprecipitation (IP)

0.5-4.0 µg

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

mDia1 Fusion Protein Ag14523 (1211-1272 aa encoded by BC007411)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules.

Immunogen sequence: AFRRKRGPR QANRKAGCAV TSLLASELTK DDAMAAVPAK VSKNSETFPT ILEEAKELVG RAS (1211-1272 aa encoded by BC007411)

Target Information

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential contractility regulates cancer stem cell migration.

Biophysical journal

Heussner RK,Zhang H,Qian G,Baker MJ,Provenzano PP

Tue Apr 04 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy.

Clinical immunology (Orlando, Fla.)

Mei J,Cai Y,Wang H,Xu R,Zhou J,Lu J,Yang X,Pan J,Liu C,Xu J,Zhu Y

Sun Jan 01 00:00:00 UTC 2023

Dia1 coordinates differentiation and cell sorting in a stratified epithelium.

The Journal of cell biology

Harmon RM,Devany J,Gardel ML

Mon May 02 00:00:00 UTC 2022

Dia1 coordinates differentiation and cell sorting in a stratified epithelium.

The Journal of cell biology

Harmon RM,Devany J,Gardel ML

Mon May 02 00:00:00 UTC 2022

Opposing roles of ZEB1 in the cytoplasm and nucleus control cytoskeletal assembly and YAP1 activity.

Cell reports

Guo Y,Lu X,Chen Y,Clark G,Trent J,Cuatrecasas M,Emery D,Song ZH,Chariker J,Rouchka E,Postigo A,Liu Y,Dean DC

Tue Oct 04 00:00:00 UTC 2022

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

Cells

Becker KN,Pettee KM,Sugrue A,Reinard KA,Schroeder JL,Eisenmann KM

Thu May 05 00:00:00 UTC 2022

Force-dependent activation of actin elongation factor mDia1 protects the cytoskeleton from mechanical damage and promotes stress fiber repair.

Developmental cell

Valencia FR,Sandoval E,Du J,Iu E,Liu J,Plotnikov SV

Mon Dec 06 00:00:00 UTC 2021

mDia1 Assembles a Linear F-Actin Coat at Membrane Invaginations To Drive Listeria monocytogenes Cell-to-Cell Spreading.

mBio

Dhanda AS,Vogl AW,Ness F,Innocenti M,Guttman JA

Tue Dec 21 00:00:00 UTC 2021

Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.

Cancers

Pettee KM,Becker KN,Alberts AS,Reinard KA,Schroeder JL,Eisenmann KM

Wed Mar 20 00:00:00 UTC 2019

Targeting the mDia Formin-Assembled Cytoskeleton Is an Effective Anti-Invasion Strategy in Adult High-Grade Glioma Patient-Derived Neurospheres.

Cancers

Pettee KM,Becker KN,Alberts AS,Reinard KA,Schroeder JL,Eisenmann KM

Wed Mar 20 00:00:00 UTC 2019

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 UTC 2018

Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.

Molecular biology of the cell

Rana MK,Aloisio FM,Choi C,Barber DL

Fri Jun 15 00:00:00 UTC 2018

Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).

PloS one

Dvorak KM,Pettee KM,Rubinic-Minotti K,Su R,Nestor-Kalinoski A,Eisenmann KM

Tue Jul 24 00:00:00 UTC 2018

Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2).

PloS one

Dvorak KM,Pettee KM,Rubinic-Minotti K,Su R,Nestor-Kalinoski A,Eisenmann KM

Tue Jul 24 00:00:00 UTC 2018

Small-molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.

Molecular biology of the cell

Arden JD,Lavik KI,Rubinic KA,Chiaia N,Khuder SA,Howard MJ,Nestor-Kalinoski AL,Alberts AS,Eisenmann KM

Sun Nov 01 00:00:00 UTC 2015

Small-molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.

Molecular biology of the cell

Arden JD,Lavik KI,Rubinic KA,Chiaia N,Khuder SA,Howard MJ,Nestor-Kalinoski AL,Alberts AS,Eisenmann KM

Sun Nov 01 00:00:00 UTC 2015

Small-molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.

Molecular biology of the cell

Arden JD,Lavik KI,Rubinic KA,Chiaia N,Khuder SA,Howard MJ,Nestor-Kalinoski AL,Alberts AS,Eisenmann KM

Sun Nov 01 00:00:00 UTC 2015

Small-molecule agonists of mammalian Diaphanous-related (mDia) formins reveal an effective glioblastoma anti-invasion strategy.

Molecular biology of the cell

Arden JD,Lavik KI,Rubinic KA,Chiaia N,Khuder SA,Howard MJ,Nestor-Kalinoski AL,Alberts AS,Eisenmann KM

Sun Nov 01 00:00:00 UTC 2015

A novel role for p115RhoGEF in regulation of epithelial plasticity.

PloS one

Kher SS,Struckhoff AP,Alberts AS,Worthylake RA

Tue Nov 11 00:00:00 UTC 2014

A novel role for p115RhoGEF in regulation of epithelial plasticity.

PloS one

Kher SS,Struckhoff AP,Alberts AS,Worthylake RA

Tue Nov 11 00:00:00 UTC 2014

An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.

PloS one

Pettee KM,Dvorak KM,Nestor-Kalinoski AL,Eisenmann KM

Tue Oct 28 00:00:00 UTC 2014

An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids.

PloS one

Pettee KM,Dvorak KM,Nestor-Kalinoski AL,Eisenmann KM

Tue Oct 28 00:00:00 UTC 2014

Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.

Circulation research

Touré F,Fritz G,Li Q,Rai V,Daffu G,Zou YS,Rosario R,Ramasamy R,Alberts AS,Yan SF,Schmidt AM

Fri May 11 00:00:00 UTC 2012

Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.

Circulation research

Touré F,Fritz G,Li Q,Rai V,Daffu G,Zou YS,Rosario R,Ramasamy R,Alberts AS,Yan SF,Schmidt AM

Fri May 11 00:00:00 UTC 2012

Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.

Circulation research

Touré F,Fritz G,Li Q,Rai V,Daffu G,Zou YS,Rosario R,Ramasamy R,Alberts AS,Yan SF,Schmidt AM

Fri May 11 00:00:00 UTC 2012

Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.

Circulation research

Touré F,Fritz G,Li Q,Rai V,Daffu G,Zou YS,Rosario R,Ramasamy R,Alberts AS,Yan SF,Schmidt AM

Fri May 11 00:00:00 UTC 2012

Formin mDia1 mediates vascular remodeling via integration of oxidative and signal transduction pathways.

Circulation research

Touré F,Fritz G,Li Q,Rai V,Daffu G,Zou YS,Rosario R,Ramasamy R,Alberts AS,Yan SF,Schmidt AM

Fri May 11 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: diaphanous 1; diaphanous homolog 1; diaphanous-1; diaphanous-related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; mDIA1; p140DIA; p140mDIA; Protein diaphanous homolog 1

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Gene Aliases: D18Wsu154e; DFNA1; DIA1; DIAP1; DIAPH1; DRF1; hDIA1; LFHL1; p140mDia; SCBMS

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UniProt ID: (Human) O60610, (Mouse) O08808

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Entrez Gene ID: (Human) 1729, (Mouse) 13367, (Rat) 307483

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actin binding receptor binding protein binding Rho GTPase binding ion channel binding poly(A) RNA binding molecular_function profilin binding identical protein binding

cytoskeleton organization sensory perception of sound regulation of cell shape actin filament polymerization positive regulation of cell migration regulation of microtubule-based process protein localization to microtubule regulation of release of sequestered calcium ion into cytosol cellular response to histamine regulation of cell motility actin cytoskeleton organization neuron projection development

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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