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mGluR2 Antibody (MA5-42460) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of mGluR2 in SH-SY-5Y cells (green) using mGluR2 Recombinant Monoclonal Antibody (Product # MA5-42460). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-42460
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JJ091-09
Immunogen
Recombinant protein within human mglur2 aa 750-872
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911601
Product Specific Information
Positive Control: SH-SY-5Y, SW480, Hela, mouse brain tissue.
Subcellular Location: Cell membrane, Cell junction, Cell projection.
Target Information
Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainite receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors stimulate phospholipase C as revealed by an increase in phosphoinositide turnover and calcium release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein coupled receptor, family C, group 1, member B; G protein-coupled receptor, family C, group 1, member B; glutamate receptor; glutamate receptor homolog; glutamate receptor, metabotropic 2; M mGlu2; Metabotropic; Metabotropic glutamate receptor 2; mGluR2
Gene Aliases: 4930441L02Rik; GLUR2; GPRC1B; GRM2; mGlu2; MGLUR2; mGluR7
UniProt ID: (Human) Q14416, (Mouse) Q14BI2, (Rat) P31421
Entrez Gene ID: (Human) 2912, (Mouse) 108068, (Rat) 24415
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway
G-protein coupled glutamate receptor signaling pathway
chemical synaptic transmission
glutamate secretion
regulation of synaptic transmission, glutamatergic
negative regulation of adenylate cyclase activity
regulation of neurotransmitter secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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