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Invitrogen
mazF Polyclonal Antibody
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mazF Antibody (PA5-144386) in WB
Western blot analysis of mazF in recombinant protein using a dilution of 1:2,000. Samples were incubated with mazF polyclonal antibody (Product # PA5-144386). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 29 kDa. Observed band size: 29 kDa.
Product Details
PA5-144386
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Escherichia coli O157:H7 mazF protein (1-111aa )
Conjugate
Form
Liquid
Concentration
4 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091634
Target Information
Toxic component of a type II toxin-antitoxin (TA) system. MazF is a sequence-specific endoribonuclease that inhibits protein synthesis and induces bacterial stasis. It is very stable, single-strand specific and cleavage is independent of the ribosome. The endoribonuclease activity (a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: chpA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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