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Invitrogen
mreB Polyclonal Antibody
FIGURE: 1 / 1
mreB Antibody (PA5-144554) in WB
Western blot analysis of mreB in recombinant protein using a dilution of 1:1,000. Samples were incubated with mreB polyclonal antibody (Product # PA5-144554). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 52 kDa. Observed band size: 52 kDa.
Product Details
PA5-144554
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Escherichia coli (strain K12) mreB protein (1-347aa)
Conjugate
Form
Liquid
Concentration
2.355 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3091665
Target Information
Forms membrane-associated dynamic filaments that are essential for cell shape determination (PubMed:15612918, PubMed:21903929). Acts by regulating cell wall synthesis and cell elongation, and thus cell shape (PubMed:21903929). A feedback loop between cell geometry and MreB localization maintains elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature (PubMed:24550515). Filaments rotate around the cell circumference in concert with the cell wall synthesis enzymes. The process is driven by the cell wall synthesis machinery and does not depend on MreB polymerization (PubMed:21903929). Rotation may contribute to the robust maintenance of rod shape (PubMed:21903929).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: envB; rodY
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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