Invitrogen

n-Myc Polyclonal Antibody

FIGURE: 1 / 4

n-Myc Antibody (PA5-17403) in WB

Western blot analysis of N-Myc in extracts from HeLa cells, either mock transfected or transfected with murine N-Myc, using N-Myc polyclonal antibody (Product # PA5-17403).

Product Image Gallery

n-Myc Antibody — Figure 5 CD532 inhibits Aurora A kinase activity and downregulates MYCN. (A-D) SKN-BE(2) cells were treated for 6 hr with the indicated compounds at 1 muM and EdU was added 1 hr prior to harvest to measure cell cycle by EdU incorporation and propidium iodide (PI) staining (A), p-H3 (B), pan-Aurora (A, B, and C isoforms) phosphorylation (C), and MYCN protein (D) by flow cytometry. See also Figure S3 . {TM}

Product Details

PA5-17403

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues surrounding lysine 351 of human N-Myc

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

31.1 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10988360

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other Myc family members.

Target Information

N-Myc protein is member of the MYC family of transcription factor proteins with a basic helix-loop-helix (bHLH) domain. N-Myc is similarly regulated and functionally complementary to C-Myc in the context of various cellular growth and differentiation processes. MYCN gene is considered the hallmark of neuroblastoma, amplification and overexpression of the MYCN proto-oncogene occurs in approximately 20% of neuroblastomas and is associated with advanced stage disease, rapid tumor progression, and poor prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nifurtimox reduces N-Myc expression and aerobic glycolysis in neuroblastoma.

Cancer biology & therapy

Cabanillas Stanchi KM,Bruchelt G,Handgretinger R,Holzer U

PA5-17403 was used in western blot to investigate the effect of nifurtimox on tumor metabolism

Wed Jul 06 00:00:00 UTC 2016

Orai1 expression pattern in tooth and craniofacial ectodermal tissues and potential functions during ameloblast differentiation.

Developmental dynamics : an official publication of the American Association of Anatomists

Zheng L,Zinn V,Lefkelidou A,Taqi N,Chatzistavrou X,Balam T,Nervina J,Papagerakis S,Papagerakis P

PA5-17403 was used in western blot to study the role of Orai1 in amelogenesis

Thu Oct 01 00:00:00 UTC 2015

Drugging MYCN through an allosteric transition in Aurora kinase A.

Cancer cell

Gustafson WC,Meyerowitz JG,Nekritz EA,Chen J,Benes C,Charron E,Simonds EF,Seeger R,Matthay KK,Hertz NT,Eilers M,Shokat KM,Weiss WA

PA5-17403 was used in flow cytometry to identify and characterize MYCN protein inhibitors

Mon Sep 08 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: bHLHe37; Class E basic helix-loop-helix protein 37; N-myc proto-oncogene protein; neuroblastoma MYC oncogene; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene; oncogene NMYC; oncogene pp65/67; pp65/67

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Gene Aliases: BHLHE37; MODED; MYCN; N-myc; NMYC; ODED

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UniProt ID: (Human) P04198

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Entrez Gene ID: (Human) 4613

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Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein dimerization activity

Process(es)

cartilage condensation positive regulation of mesenchymal cell proliferation transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of gene expression positive regulation of cell death lung development embryonic digit morphogenesis regulation of auditory receptor cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic skeletal system morphogenesis negative regulation of astrocyte differentiation branching morphogenesis of an epithelial tube negative regulation of reactive oxygen species metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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n-Myc Polyclonal Antibody

n-Myc Antibody (PA5-17403) in WB

Product Details

PA5-17403

Western Blot (WB)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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