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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • nNOS Antibodies

          Invitrogen

          nNOS Polyclonal Antibody

          39 Published Figures
          85 References
          View all (25) nNOS antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite nNOS Polyclonal Antibody

          • Antibody Testing Data (2)
          • Published Figures (39)
          nNOS Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Group 53 Created with Sketch.
          nNOS Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Group 53 Created with Sketch.

          FIGURE: 1 / 41

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          nNOS Antibody (61-7000) in IHC (F)

          Immunohistochemical staining of frozen rat cerebral cortex using Rb anti-nNOS (Product # 61-7000). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          nNOS Antibody in Immunohistochemistry (Frozen) (IHC (F))
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          nNOS Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          nNOS Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          nNOS Antibody in Immunocytochemistry (ICC/IF)
          nNOS Antibody in Immunocytochemistry (ICC/IF)
          nNOS Antibody in Immunocytochemistry (ICC/IF)
          nNOS Antibody in Immunocytochemistry (ICC/IF)
          nNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          nNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          nNOS Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          nNOS Antibody in Western Blot, Immunohistochemistry (Frozen) (WB, IHC (F))
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry, Immunohistochemistry - Free Floating (IHC, IHC (Free))
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry (IHC)
          nNOS Antibody in Immunohistochemistry - Free Floating (IHC (Free))
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot (WB)
          nNOS Antibody in Western Blot, Immunoprecipitation (WB, IP)
          nNOS Antibody in Immunomicroscopy (IM)
          nNOS Antibody in Immunomicroscopy (IM)

          Product Details

          61-7000

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 13 publications 13 publications

          Immunohistochemistry (IHC)

          -
          View 50 publications 50 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          5-10 µg/mL
          View 6 publications 6 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1-2 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry - Free Floating (IHC (Free))

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          -
          View 8 publications 8 publications

          ELISA (ELISA)

          0.1-1 µg/mL
          -

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Immunomicroscopy (IM)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A recombinant protein consisting of 195 amino acids from the N-terminus of rat nNOS protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.25 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.4

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2313734

          Product Specific Information

          This antibody reacts with the ~160 kDa nNOS protein and does not exhibit any cross-reactivity with the related eNOS or iNOS proteins. During development reactivity was confirmed with a ~160 kD band on western blots of rat and mouse brain tissue lysates (20 µg). For use in immunohistochemistry with paraffin embedded tissues some background staining was evident.

          Target Information

          NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: bNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; Nitric oxide synthase 1; Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS

          View more View less

          Gene Aliases: 2310005C01Rik; Bnos; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; NOS-I; Nos1

          View more View less

          UniProt ID: (Rat) P29476, (Mouse) Q9Z0J4

          View more View less

          Entrez Gene ID: (Rat) 24598, (Mouse) 18125

          View more View less

          Function(s)
          NADPH-hemoprotein reductase activity nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding amino acid binding sodium channel regulator activity enzyme binding heme binding protein homodimerization activity ion channel binding cadmium ion binding flavin adenine dinucleotide binding NADP binding ATPase binding scaffold protein binding
          Process(es)
          response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process muscle contraction striated muscle contraction nitric oxide mediated signal transduction female pregnancy aging regulation of heart contraction negative regulation of cell proliferation response to heat response to organonitrogen compound response to lead ion response to organic cyclic compound response to activity peptidyl-cysteine S-nitrosylation positive regulation of guanylate cyclase activity response to nutrient levels response to lipopolysaccharide negative regulation of peptidyl-serine phosphorylation response to vitamin E multicellular organismal response to stress positive regulation of histone acetylation response to nicotine exogenous drug catabolic process negative regulation of apoptotic process negative regulation of potassium ion transport response to peptide hormone response to estrogen response to ethanol negative regulation of blood pressure negative regulation of heart contraction positive regulation of transcription, DNA-templated negative regulation of vasoconstriction positive regulation of vasodilation positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of sensory perception of pain oxidation-reduction process cellular response to mechanical stimulus cellular response to growth factor stimulus response to nitric oxide cellular response to epinephrine stimulus positive regulation of the force of heart contraction positive regulation of long-term synaptic potentiation positive regulation of adrenergic receptor signaling pathway involved in heart process positive regulation of neuron death positive regulation of sodium ion transmembrane transport
          It has to be done as per old AB suggested Products section.
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