Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • p19ARF Antibodies

          Invitrogen

          p19ARF Polyclonal Antibody

          8 Published Figures
          9 References
          View all (8) p19ARF antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite p19ARF Polyclonal Antibody

          • Antibody Testing Data (3)
          • Published Figures (8)
          p19ARF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          p19ARF Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          p19ARF Antibody (PA1-30670) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of p19ARF in MEF cells using p19ARF Polyclonal Antibody (Product # PA1-30670) (Green). Red : Tubulin. Blue: DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          p19ARF Antibody in Immunocytochemistry (ICC/IF)
          p19ARF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          p19ARF Antibody in Western Blot (WB)
          p19ARF Antibody in Immunohistochemistry (IHC)
          p19ARF Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          p19ARF Antibody in Immunohistochemistry (IHC)
          p19ARF Antibody in Immunohistochemistry (IHC)
          p19ARF Antibody in Western Blot (WB)
          p19ARF Antibody in Immunoprecipitation (IP)
          p19ARF Antibody in ChIP Assay (ChIP)
          p19ARF Antibody in ChIP Assay (ChIP)

          Product Details

          PA1-30670

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2 µg/mL
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          10 µg/mL
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:100
          View 1 publication 1 publication

          ELISA (ELISA)

          1:100-1:2,000
          -

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide conjugated to KLH, derived from a sequence within residues 50-150 of mouse p19ARF..
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          tris citrate/tris phosphate, pH 7.0-8.0

          Contains

          0.1% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1956784

          Product Specific Information

          PA1-30670 recognizes p19ARF, Uniprot ID Q64364.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Alternative reading frame; ARF; CDK4I; cdkn2a; cdkn2a {ECO:0000312|EMBL:AAL76336.1, ECO:0000312|RGD:2323}; cdkn2a {ECO:0000312|EMBL:AAL76338.1, ECO:0000312|RGD:2323}; cell cycle inhibitor; cell cycle regulator; Cyclin dependent kinase inhibitor 2A (p16, inhibits CDK4); Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2a p16Ink4a; cyclin-dependent kinase inhibitor 2a p19Arf; cyclin-dependent kinase inhibitor 2A, isoform 1; cyclin-dependent kinase inhibitor 2A, isoform 2; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; mitochondrial smARF; p16; p16-INK4; p16-INK4a; p19ARF; Tumor suppressor ARF

          View more View less

          Gene Aliases: Arf; ARF-INK4a; Cdkn2a; INK4A; INK4a-ARF; Ink4a/Arf; MTS1; p16; p16(INK4a); p16Cdkn2a; P16ink4a; p19; p19ARF; Pctr1

          View more View less

          UniProt ID: (Mouse) P51480, (Rat) Q9R0Z3

          View more View less

          Entrez Gene ID: (Mouse) 12578, (Rat) 25163

          View more View less

          Function(s)
          p53 binding DNA binding transcription factor activity, sequence-specific DNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding transcription factor binding protein kinase binding poly(A) RNA binding protein N-terminus binding NF-kappaB binding ubiquitin-protein transferase inhibitor activity MDM2/MDM4 family protein binding
          Process(es)
          regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle protein polyubiquitination mitophagy negative regulation of cell-matrix adhesion transcription, DNA-templated regulation of transcription, DNA-templated rRNA processing negative regulation of protein kinase activity apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cell cycle cell cycle arrest aging cell aging negative regulation of cell proliferation epidermis development apoptotic mitochondrial changes rRNA transcription response to organonitrogen compound regulation of G2/M transition of mitotic cell cycle regulation of gene expression response to organic cyclic compound negative regulation of cell growth negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of NF-kappaB transcription factor activity negative regulation of protein binding negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process somatic stem cell population maintenance senescence-associated heterochromatin focus assembly negative regulation of phosphorylation response to drug glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of ubiquitin-protein transferase activity mitochondrial depolarization positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination positive regulation of cell cycle arrest cellular senescence replicative senescence positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator regulation of apoptotic DNA fragmentation negative regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process positive regulation of cellular senescence Ras protein signal transduction response to organic substance cerebellum development
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67f4cd694f-jv9gd:80/100.66.135.11:80.
          git-commit: 961b7666e63bcc533d6299fdd217d6a70bd96cf1
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.32.0-2025.07.28-1.0