Proteintech

p21 Monoclonal Antibody (3C7D3)

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View all (83) p21 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

67362-1-IG

Applications
Tested Dilution
Publications

Western Blot (WB)

1:3,000
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:100
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3C7D3

Immunogen

Cdkn1a Fusion Protein Ag28394

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1000 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; Melanoma differentiation-associated protein; OTTHUMP00000016298; p21; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1)

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Gene Aliases: CAP20; CDKI; Cdkn1; Cdkn1a; CIP1; mda6; P21; p21Cip1; p21WAF; SDI1; Waf1

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UniProt ID: (Mouse) P39689

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Entrez Gene ID: (Mouse) 12575

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Function(s)
protein kinase inhibitor activity cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle protein phosphorylation cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle cell cycle arrest regulation of mitotic cell cycle negative regulation of cell proliferation response to UV negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation cellular response to extracellular stimulus regulation of protein import into nucleus, translocation negative regulation of phosphorylation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of protein kinase activity positive regulation of fibroblast proliferation regulation of cell cycle cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest signal transduction by p53 class mediator cellular senescence negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process
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