Invitrogen

p21 Monoclonal Antibody (GT8611)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-31480

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT8611

Immunogen

Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787111

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Cat (84%), Sheep (82%), Rhesus Monkey (98%), Bovine (83%).

Positive Control: HeLa, HCT116 (30 µM Cisplatin treatment for 24 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; PIC1; SDI1; WAF1

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UniProt ID: (Human) P38936

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Entrez Gene ID: (Human) 1026

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Function(s)
cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction negative regulation of cell proliferation response to toxic substance response to X-ray response to organonitrogen compound negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation organ regeneration cellular response to extracellular stimulus regulation of protein import into nucleus, translocation cellular response to amino acid starvation cellular response to heat negative regulation of phosphorylation response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity response to arsenic-containing substance positive regulation of fibroblast proliferation protein stabilization response to corticosterone response to hyperoxia intestinal epithelial cell maturation cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest cellular senescence replicative senescence stress-induced premature senescence intrinsic apoptotic signaling pathway positive regulation of cyclin-dependent protein kinase activity negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process
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