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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • p21 Antibodies

          Invitrogen

          p21 (WAF1, Cip1) Polyclonal Antibody, eBioscience™

          13 Published Figures
          12 References
          View all (83) p21 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite p21 (WAF1, Cip1) Polyclonal Antibody, eBioscience™

          • Antibody Testing Data (2)
          • Published Figures (13)
          p21 (WAF1, Cip1) Antibody in Immunocytochemistry (ICC/IF)
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          p21 (WAF1, Cip1) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          p21 (WAF1, Cip1) Antibody (14-6715-81) in ICC/IF

          Immunofluorescence analysis of p21 (WAF1,Cip1) was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p21 (WAF1,Cip1) Rabbit Polyclonal Antibody (Product # 14-6715-81) at 5 microgram/mL in 0.1% BSA, incubated... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          p21 (WAF1, Cip1) Antibody in Immunocytochemistry (ICC/IF)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Immunocytochemistry (ICC/IF)
          p21 (WAF1, Cip1) Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)
          p21 (WAF1, Cip1) Antibody in Western Blot (WB)

          Product Details

          14-6715-81

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          Assay-Dependent
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS

          Contains

          0.1% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_468227

          Product Specific Information

          Description: The polyclonal antibody reacts with mouse, rat, and human p21; the antibody was raised against a peptide mapping to the carboxy terminus of human p21. The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

          Applications Reported: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

          Applications Tested: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been tested by immunoblotting (WB) (1:200 starting dilution).

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

          View more View less

          Gene Aliases: CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; UV96; WAF1

          View more View less

          UniProt ID: (Human) P38936, (Mouse) P39689

          View more View less

          Entrez Gene ID: (Human) 1026, (Mouse) 12575, (Rat) 114851

          View more View less

          Function(s)
          cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding protein kinase inhibitor activity
          Process(es)
          regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction negative regulation of cell proliferation response to toxic substance response to X-ray response to organonitrogen compound negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation organ regeneration cellular response to extracellular stimulus regulation of protein import into nucleus, translocation cellular response to amino acid starvation cellular response to heat negative regulation of phosphorylation response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity response to arsenic-containing substance positive regulation of fibroblast proliferation protein stabilization response to corticosterone response to hyperoxia intestinal epithelial cell maturation cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest cellular senescence replicative senescence stress-induced premature senescence intrinsic apoptotic signaling pathway positive regulation of cyclin-dependent protein kinase activity negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process protein phosphorylation cell cycle regulation of mitotic cell cycle response to UV positive regulation of protein kinase activity regulation of cell cycle signal transduction by p53 class mediator response to organic substance response to organic cyclic compound response to glucocorticoid
          It has to be done as per old AB suggested Products section.
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