Invitrogen

p21 (WAF1, Cip1) Polyclonal Antibody, eBioscience™

13 Published Figures

12 References

View all (83) p21 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (13)

p21 (WAF1, Cip1) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

p21 (WAF1, Cip1) Antibody (14-6715-81) in ICC/IF

Immunofluorescence analysis of p21 (WAF1,Cip1) was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p21 (WAF1,Cip1) Rabbit Polyclonal Antibody (Product # 14-6715-81) at 5 microgram/mL in 0.1% BSA, incubated... View More

p21 (WAF1, Cip1) Antibody in Western Blot (WB)

p21 (WAF1, Cip1) Antibody in Western Blot, Immunohistochemistry (Paraffin) (WB, IHC (P))

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Product Details

14-6715-81

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

View 6 publications 6 publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468227

Product Specific Information

Description: The polyclonal antibody reacts with mouse, rat, and human p21; the antibody was raised against a peptide mapping to the carboxy terminus of human p21. The progression through the cell cycle is regulated by cyclins and their cognate Cdks by promoting cell cycle transitions (1,2,3). This orderly progression can be inhibited by a family of proteins known as CDK inhibitors (CDIs) that bind to cyclin/Cdk complexes and halt cell division (3). p21 (also designated WAF1/Cip1) is one has been shown to inhibit the activity of each member of the cyclin/Cdk family and over expression of this protein inhibits the proliferation of mammalian cells (5). The expression of p21 is inducible by a wide range of stress stimuli and its transcription can be enhanced by p53 (6). Another member of the CDIs is p27 (also known as Kip1), which also sees up regulation in response to antimitogenic stimuli (7). The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes, like cyclin D1/Cdk4 (4,8). An additional CDI has been found to bind Cdk4 and Cdk6, p16 (INK4A), and when such complexes are formed, the progression of the cell cycle is halted (9). It has become increasingly clear that p16 is a very important tumor suppressor gene whose frequent loss occurs early in many human cancers. p16 is a major target in carcinogenesis that is rivaled in frequency only by p53 (10).

Applications Reported: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: Purified anti-mo/hu/rat p21 (WAF1/Cip1) poly has been tested by immunoblotting (WB) (1:200 starting dilution).

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. P21 is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in the nucleus executes binding and inhibition activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage, which enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. P21 is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 exhibits a dual function in carcinogenesis, and acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183.

Scientific reports

Di Luccia B,Acampora V,Saggese A,Calabrò V,Vivo M,Angrisano T,Baccigalupi L,Ricca E,Pollice A

14-6715-81 was used in Western Blotting to show that bioactive molecules secreted by SF1183 reduce the proliferation of HCT116 cells in a reversible manner determining a variation in cell cycle markers (p21WAF, p53, cyclin D1) and resulting in the protection of HCT116 cells from TNF-alfa induced apoptosis, an effect potentially relevant for the protection of the epithelial barrier integrity and reconstitution of tissue homeostasis.

Thu Nov 24 00:00:00 UTC 2022

Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183.

Scientific reports

Di Luccia B,Acampora V,Saggese A,Calabrò V,Vivo M,Angrisano T,Baccigalupi L,Ricca E,Pollice A

Thu Nov 24 00:00:00 UTC 2022

Targeting p21^Cip1 highly expressing cells in adipose tissue alleviates insulin resistance in obesity.

Cell metabolism

Wang L,Wang B,Gasek NS,Zhou Y,Cohn RL,Martin DE,Zuo W,Flynn WF,Guo C,Jellison ER,Kim T,Prata LGPL,Palmer AK,Li M,Inman CL,Barber LS,Al-Naggar IMA,Zhou Y,Du W,Kuchel GA,Meves A,Tchkonia T,Kirkland JL,Robson P,Xu M

14-6715-81 was used in Flow cytometry/Cell sorting to establish that p21high cells within fat are sufficient to cause insulin resistance in vivo.

Tue Jan 04 00:00:00 UTC 2022

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Bisphenol A Induces Accelerated Cell Aging in Murine Endothelium.

Biomolecules

Moreno-Gómez-Toledano R,Sánchez-Esteban S,Cook A,Mínguez-Moratinos M,Ramírez-Carracedo R,Reventún P,Delgado-Marín M,Bosch RJ,Saura M

14-6715-81 was used in Western Blotting and Immunohistochemistry to evaluate the possible involvement of BPA-induced senescence mechanisms in endothelial cells.

Wed Sep 29 00:00:00 UTC 2021

Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells.

Nature communications

Jia W,Kong L,Kidoya H,Naito H,Muramatsu F,Hayashi Y,Hsieh HY,Yamakawa D,Hsu DK,Liu FT,Takakura N

Published figure using p21 (WAF1, Cip1) polyclonal antibody (Product # 14-6715-81) in Immunocytochemistry

Fri Apr 09 00:00:00 UTC 2021

Sympathetic Denervation Ameliorates Renal Fibrosis via Inhibition of Cellular Senescence.

Frontiers in immunology

Li Q,Deng Y,Liu L,Zhang C,Cai Y,Zhang T,Han M,Xu G

14-6715-81 was used in Western Blotting to test the hypothesis that sympathetic activation in the injured kidney induces cellular senescence, which contributes to progressive renal inflammation and fibrosis.

Thu Mar 03 00:00:00 UTC 2022

Sympathetic Denervation Ameliorates Renal Fibrosis via Inhibition of Cellular Senescence.

Frontiers in immunology

Li Q,Deng Y,Liu L,Zhang C,Cai Y,Zhang T,Han M,Xu G

Thu Mar 03 00:00:00 UTC 2022

Upregulation of Chemoresistance by Mg²⁺ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.

Cells

Onuma S,Manabe A,Yoshino Y,Matsunaga T,Asai T,Ikari A

14-6715-81 was used in Western Blot to investigate the effect of low Mg2+ concentration (LM) on proliferation and chemosensitivity using human lung adenocarcinoma A549 cells.

Wed May 12 00:00:00 UTC 2021

Upregulation of Chemoresistance by Mg²⁺ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells.

Cells

Onuma S,Manabe A,Yoshino Y,Matsunaga T,Asai T,Ikari A

14-6715-81 was used in Western Blot to investigate the effect of low Mg2+ concentration (LM) on proliferation and chemosensitivity using human lung adenocarcinoma A549 cells.

Wed May 12 00:00:00 UTC 2021

An inducible p21-Cre mouse model to monitor and manipulate p21-highly-expressing senescent cells in vivo.

Nature aging

Wang B,Wang L,Gasek NS,Zhou Y,Kim T,Guo C,Jellison ER,Haynes L,Yadav S,Tchkonia T,Kuchel GA,Kirkland JL,Xu M

14-6715-81 was used in Immunocytochemistry-immunofluorescence to demonstrate that the p21-Cre mouse model is a valuable and powerful tool for studying p21 high cells to further understand the biology of senescent cells.

Fri Oct 01 00:00:00 UTC 2021

CRISPR/Cas9 treatment causes extended TP53-dependent cell cycle arrest in human cells.

Nucleic acids research

Geisinger JM,Stearns T

14-6715-63 was used in Immunocytochemistry to test a hypothesis that the basis for the large variation in mutant recovery for a given target sequence between cell lines may be due to differences in how the DNA damage response affects cell cycle progression.

Fri Sep 18 00:00:00 UTC 2020

Delayed effects of acute whole body lethal radiation exposure in mice pre-treated with BBT-059.

Scientific reports

Sharma NK,Holmes-Hampton GP,Kumar VP,Biswas S,Wuddie K,Stone S,Aschenake Z,Wilkins WL,Fam CM,Cox GN,Ghosh SP

14-6715-81 was used in Western Blotting to describe the use of a promising radiation countermeasure, BBT-059, and the results of a long term mouse study (up to 12 months) in the male CD2F1 strain using 60Co gamma irradiation (~0.6 Gy/min, 7.5-12.5 Gy).

Wed Apr 22 00:00:00 UTC 2020

Delayed effects of acute whole body lethal radiation exposure in mice pre-treated with BBT-059.

Scientific reports

Sharma NK,Holmes-Hampton GP,Kumar VP,Biswas S,Wuddie K,Stone S,Aschenake Z,Wilkins WL,Fam CM,Cox GN,Ghosh SP

Wed Apr 22 00:00:00 UTC 2020

An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7.

PloS one

Ghate NB,Chaudhuri D,Sarkar R,Sajem AL,Panja S,Rout J,Mandal N

14-6715 was used in Western Blotting to hypothesise that the antioxidant and anticancer potential of the PRME may reside in the phytoconstitutents present in it.

Tue Sep 30 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Activating Fragment 1; CDK-interacting protein 1; CDK-interaction protein 1; CDNK1A; Cip1); Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (p21; cyclin-dependent kinase inhibitor 1A (P21); cyclin-dependent kinase inhibitor 1A (p21, Cip1); cyclin-dependent kinase inhibitor 1A variant 1; cyclin-dependent kinase inhibitor 1A variant 2; DNA synthesis inhibitor; MDA-6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein; Melanoma differentiation-associated protein 6; OTTHUMP00000016298; p21; p21; p21Cip1/Waf1; SLC12A9; Wild type p53 activated fragment 1 (WAF1); wild-type p53-activated fragment 1

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Gene Aliases: CAP20; CDKI; CDKN1; CDKN1A; CIP1; MDA-6; MDA6; P21; p21CIP1; p21WAF; PIC1; SDI1; UV96; WAF1

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UniProt ID: (Human) P38936, (Mouse) P39689

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Entrez Gene ID: (Human) 1026, (Mouse) 12575, (Rat) 114851

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Function(s)

cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding cyclin-dependent protein kinase activating kinase activity cyclin binding ubiquitin protein ligase binding protein complex binding metal ion binding protein kinase inhibitor activity

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Ras protein signal transduction negative regulation of cell proliferation response to toxic substance response to X-ray response to organonitrogen compound negative regulation of gene expression negative regulation of cell growth positive regulation of B cell proliferation organ regeneration cellular response to extracellular stimulus regulation of protein import into nucleus, translocation cellular response to amino acid starvation cellular response to heat negative regulation of phosphorylation response to drug intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of apoptotic process positive regulation of programmed cell death negative regulation of cyclin-dependent protein serine/threonine kinase activity response to arsenic-containing substance positive regulation of fibroblast proliferation protein stabilization response to corticosterone response to hyperoxia intestinal epithelial cell maturation cellular response to ionizing radiation cellular response to UV-B mitotic cell cycle arrest cellular senescence replicative senescence stress-induced premature senescence intrinsic apoptotic signaling pathway positive regulation of cyclin-dependent protein kinase activity negative regulation of G1/S transition of mitotic cell cycle regulation of DNA biosynthetic process positive regulation of reactive oxygen species metabolic process protein phosphorylation cell cycle regulation of mitotic cell cycle response to UV positive regulation of protein kinase activity regulation of cell cycle signal transduction by p53 class mediator response to organic substance response to organic cyclic compound response to glucocorticoid

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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p21 (WAF1, Cip1) Polyclonal Antibody, eBioscience™

p21 (WAF1, Cip1) Antibody (14-6715-81) in ICC/IF

Product Details

14-6715-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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