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Invitrogen
p23 Polyclonal Antibody
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FIGURE: 1 / 8
p23 Antibody (PA5-28476) in ICC/IF
Immunofluorescence analysis of p23 was performed using RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with p23 Rabbit Polyclonal Antibody (Product # PA5-28476) at 5µg/mL dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of ... View More
Product Details
PA5-28476
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human p23. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545952
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain.
Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (84%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; Cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; p23 cochaperone; Progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); Sid 3177; telomerase binding protein, p23; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD
Gene Aliases: 5730442A20Rik; cPGES; P23; Ptges; PTGES3; Sid3177; TEBP
UniProt ID: (Human) Q15185, (Mouse) Q9R0Q7
Entrez Gene ID: (Human) 10728, (Mouse) 56351
telomere maintenance
prostaglandin biosynthetic process
RNA-dependent DNA biosynthetic process
signal transduction
cyclooxygenase pathway
chaperone cofactor-dependent protein refolding
regulation of cellular response to heat
glycogen biosynthetic process
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
prostaglandin metabolic process
cell proliferation
positive regulation of gene expression
sensory perception of pain
glucocorticoid receptor signaling pathway
skin development
lung saccule development
negative regulation of cell death
DNA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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