Invitrogen

p23 Monoclonal Antibody (JJ3)

p23 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

p23 Antibody (MA3-414) in ICC/IF

Immunofluorescent analysis of p23 using Anti-p23 Monoclonal Antibody (JJ5) (Product # MA3-414) shows staining in Hela Cells. p23 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing p23 (Product # MA3-414) at a dilution of 1:500 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat ... View More

p23 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

p23 Antibody in Immunoprecipitation (IP)

p23 Antibody in RNA Immunoprecipitation (RIP)

p23 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

p23 Antibody in Immunohistochemistry (IHC)

p23 Antibody in Proximity Ligation Assay (PLA) (PLA)

Product Image Gallery

p23 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. T-47D cells were transfected with p23 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-p23 Monoclonal Antibody (JJ3) (Product # MA3-414). {KD}

Product Details

MA3-414

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:200-1:2,000

View 4 publications 4 publications

Flow Cytometry (Flow)

1/100

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

in situ PLA (PLA)

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

Product Specifications

Species Reactivity

Chicken, Guinea pig, Human, Mouse, Rabbit, Rat

Published species

Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

JJ3

Immunogen

Recombinant human p23 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2175197

Product Specific Information

MA3-414 detects p23 from chicken, guinea pig, human, rat, mouse and rabbit samples.

MA3-414 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 19 or 23 kDa protein representing p23 from rabbit reticulocyte lysate. MA3-414 can be used to immunoprecipitate both free and complexed p23.

The MA3-414 antigen is recombinant human p23.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP70 and HSP90), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and at least three other proteins termed p23, p60 and p48. p23 along with HSP70, HSP90 and p60, combine with progesterone receptor (PR) as members of a transient intermediate complex. Cloned human p23 encodes a protein of 160 amino acids that is highly conserved between species and shows no homology to previously identified proteins. p23 is a highly acidic phosphoprotein with an aspartic acid-rich C-terminal domain and multiple potential phosphorylation sites. In vitro studies have suggested that p23 binds to HSP90 and is necessary for the binding of HSP90 and CyP-40 to PR. While neither its exact function nor mechanism of action have been identified, p23 appears to be an important factor in PR function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Alpha Isoform of Heat Shock Protein 90 and the Co-chaperones p23 and Cdc37 Promote Opioid Anti-nociception in the Brain.

Frontiers in molecular neuroscience

Lei W,Duron DI,Stine C,Mishra S,Blagg BSJ,Streicher JM

MA3-414 was used in Immunohistochemistry to provide insight into the molecular mechanisms by which Hsp90 promotes opioid anti-nociception.

Fri Oct 20 00:00:00 UTC 2023

In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network.

FEBS open bio

Bernadotte A,Kumar R,Winblad B,Pavlov PF

MA3-414 was used in Immunocytochemistry-immunoflourescence to identify and characterise the human dicarboxylate clamp tetratricopeptide repeat protein interaction network.

Thu Nov 01 00:00:00 UTC 2018

In silico identification and biochemical characterization of the human dicarboxylate clamp TPR protein interaction network.

FEBS open bio

Bernadotte A,Kumar R,Winblad B,Pavlov PF

MA3-414 was used in Immunocytochemistry-immunoflourescence to identify and characterise the human dicarboxylate clamp tetratricopeptide repeat protein interaction network.

Thu Nov 01 00:00:00 UTC 2018

Hsp70 cochaperones HspBP1 and BAG-1M differentially regulate steroid hormone receptor function.

PloS one

Knapp RT,Wong MJ,Kollmannsberger LK,Gassen NC,Kretzschmar A,Zschocke J,Hafner K,Young JC,Rein T

MA3-414 was used in western blot to study the functional regulation of steroid hormone receptors by the Hsp70 co-chaperones HspBP1 and GAG-1M

Wed Sep 17 00:00:00 UTC 2014

Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells.

Molecular biology of the cell

Pare JM,LaPointe P,Hobman TC

MA3-414 was used in western blot to study the interaction of p23 and FKBP4 with hAgo2 and their roles in the mechanism of mammalian cell RNAi silencing

Thu Aug 01 00:00:00 UTC 2013

pRb controls estrogen receptor alpha protein stability and activity.

Oncotarget

Caligiuri I,Toffoli G,Giordano A,Rizzolio F

MA3-414 was used in western blot to investigate cross talk between the estrogen receptor and the retinoblastoma pathway

Sat Jun 01 00:00:00 UTC 2013

Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis.

The Journal of biological chemistry

Donnelly BF,Needham PG,Snyder AC,Roy A,Khadem S,Brodsky JL,Subramanya AR

MA3-414 was used in western blot to study the role of Hsp70 and Hsp90 in the endoplasmic reticulum quality control of the thiazide-sensitive NaCl co-transporter

Fri May 03 00:00:00 UTC 2013

p23 co-chaperone protects the aryl hydrocarbon receptor from degradation in mouse and human cell lines.

Biochemical pharmacology

Nguyen PM,Wang D,Wang Y,Li Y,Uchizono JA,Chan WK

MA3-414 was used in western blot to study the role of p23 in protecting the aryl hydrocarbon receptor against degradation

Sat Sep 15 00:00:00 UTC 2012

Type 1 inositol-1,4,5-trisphosphate receptor is a late substrate of caspases during apoptosis.

Journal of cellular biochemistry

Elkoreh G,Blais V,Béliveau E,Guillemette G,Denault JB

MA3-414 was used in western blot to study role of the type 1 inositol-1,4,5-trisphosphate receptor as an apoptotic substrate of caspases

Wed Aug 01 00:00:00 UTC 2012

Molecular determinants involved in activation of caspase 7.

Bioscience reports

Boucher D,Blais V,Drag M,Denault JB

MA3-414 was used in western blot to investigate the caspase activation pathway during apoptosis

Mon Aug 01 00:00:00 UTC 2011

Caspase-1 promiscuity is counterbalanced by rapid inactivation of processed enzyme.

The Journal of biological chemistry

Walsh JG,Logue SE,Lüthi AU,Martin SJ

MA3-414 was used in western blot to investigate the substrate specificity of caspase-1

Fri Sep 16 00:00:00 UTC 2011

High levels of Hsp90 cochaperone p23 promote tumor progression and poor prognosis in breast cancer by increasing lymph node metastases and drug resistance.

Cancer research

Simpson NE,Lambert WM,Watkins R,Giashuddin S,Huang SJ,Oxelmark E,Arju R,Hochman T,Goldberg JD,Schneider RJ,Reiz LF,Soares FA,Logan SK,Garabedian MJ

MA3-414 was used in immunohistochemistry to investigate the mechanism of Hsp90 cochaperone p23 in tumor progression and poor prognosis in breast cancer

Mon Nov 01 00:00:00 UTC 2010

High levels of Hsp90 cochaperone p23 promote tumor progression and poor prognosis in breast cancer by increasing lymph node metastases and drug resistance.

Cancer research

Simpson NE,Lambert WM,Watkins R,Giashuddin S,Huang SJ,Oxelmark E,Arju R,Hochman T,Goldberg JD,Schneider RJ,Reiz LF,Soares FA,Logan SK,Garabedian MJ

MA3-414 was used in immunohistochemistry to investigate the mechanism of Hsp90 cochaperone p23 in tumor progression and poor prognosis in breast cancer

Mon Nov 01 00:00:00 UTC 2010

Glucose deprivation induces an atypical form of apoptosis mediated by caspase-8 in Bax-, Bak-deficient cells.

Cell death and differentiation

Caro-Maldonado A,Tait SW,Ramírez-Peinado S,Ricci JE,Fabregat I,Green DR,Muñoz-Pinedo C

MA3-414 was used in western blot to investigate the mechanism of apoptosis induced by glucose deprivation in Bax- and Bak-deficient cells

Sun Aug 01 00:00:00 UTC 2010

Calpain and caspase processing of caspase-12 contribute to the ER stress-induced cell death pathway in differentiated PC12 cells.

Apoptosis : an international journal on programmed cell death

Martinez JA,Zhang Z,Svetlov SI,Hayes RL,Wang KK,Larner SF

MA3-414 was used in western blot to investigate the involvement of calpain and caspase 12 in the cell death induced by endoplasmic reticulum stress

Wed Dec 01 00:00:00 UTC 2010

Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth.

Journal of neurochemistry

Quintá HR,Maschi D,Gomez-Sanchez C,Piwien-Pilipuk G,Galigniana MD

Published figure using p23 monoclonal antibody (Product # MA3-414) in Immunocytochemistry

Mon Nov 01 00:00:00 UTC 2010

Subcellular rearrangement of hsp90-binding immunophilins accompanies neuronal differentiation and neurite outgrowth.

Journal of neurochemistry

Quintá HR,Maschi D,Gomez-Sanchez C,Piwien-Pilipuk G,Galigniana MD

Published figure using p23 monoclonal antibody (Product # MA3-414) in Immunocytochemistry

Mon Nov 01 00:00:00 UTC 2010

The hsp90-FKBP52 complex links the mineralocorticoid receptor to motor proteins and persists bound to the receptor in early nuclear events.

Molecular and cellular biology

Galigniana MD,Erlejman AG,Monte M,Gomez-Sanchez C,Piwien-Pilipuk G

MA3-414 was used in western blot to investigate the role of the heat shock protein 90-FKBP52 complex in the nucleus

Mon Mar 01 00:00:00 UTC 2010

ATP binding to Hsp90 is sufficient for effective chaperoning of p53 protein.

The Journal of biological chemistry

Walerych D,Gutkowska M,Klejman MP,Wawrzynow B,Tracz Z,Wiech M,Zylicz M,Zylicz A

MA3-414 was used in western blot to study the mechanistic role of ATP in the Hsp90-p53 interaction.

Fri Oct 15 00:00:00 UTC 2010

Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway.

Cell death and differentiation

Inoue S,Browne G,Melino G,Cohen GM

MA3-414 was used in western blot to investigate the hierarchical ordering of caspases in a more relevant physiological scenario

Wed Jul 01 00:00:00 UTC 2009

Expression of Hsp90 chaperone [corrected] proteins in human tumor tissue.

International journal of biological macromolecules

McDowell CL,Bryan Sutton R,Obermann WM

MA3-414 was used in immunohistochemistry to study the expression profiling of chaperone proteins in human tumor tissue samples

Thu Oct 01 00:00:00 UTC 2009

17-DMAG ameliorates polyglutamine-mediated motor neuron degeneration through well-preserved proteasome function in an SBMA model mouse.

Human molecular genetics

Tokui K,Adachi H,Waza M,Katsuno M,Minamiyama M,Doi H,Tanaka K,Hamazaki J,Murata S,Tanaka F,Sobue G

MA3-414 was used in western blot to study the effect of 17-(dimethylaminoethylamino)-17-demethoxygeldanamycin on polyglutamine-mediated motor neuron degeneration.

Sun Mar 01 00:00:00 UTC 2009

Nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex is mediated by its interaction with Nup62 and importin beta.

Molecular and cellular biology

Echeverría PC,Mazaira G,Erlejman A,Gomez-Sanchez C,Piwien Pilipuk G,Galigniana MD

MA3-414 was used in immunoprecipitation to study the mechanism of nuclear import of the glucocorticoid receptor-hsp90 complex through the nuclear pore complex

Tue Sep 01 00:00:00 UTC 2009

Hsp90 regulates the Fanconi anemia DNA damage response pathway.

Blood

Oda T,Hayano T,Miyaso H,Takahashi N,Yamashita T

MA3-414 was used in immunoprecipitation to study the role of Hsp90 in regulating the Fanconi anemia DNA damage response pathway

Fri Jun 01 00:00:00 UTC 2007

Multiple 40-kDa heat-shock protein chaperones function in Tom70-dependent mitochondrial import.

Molecular biology of the cell

Bhangoo MK,Tzankov S,Fan AC,Dejgaard K,Thomas DY,Young JC

MA3-414 was used in western blot to investigate the roles of multiple 40-kDa heat-shock protein chaperones during the Tom70-dependent mitochondrial import.

Sat Sep 01 00:00:00 UTC 2007

Cytosolic prostaglandin E2 synthase (cPGES) expression is decreased in discrete cortical regions in psychiatric disease.

Brain research

Maida ME,Hurley SD,Daeschner JA,Moore AH,O'Banion MK

MA3-414 was used in western blot to investigate the change of expression of cytosolic prostaglandin E2 synthase in psychiatric patients

Fri Aug 04 00:00:00 UTC 2006

17-AAG, an Hsp90 inhibitor, ameliorates polyglutamine-mediated motor neuron degeneration.

Nature medicine

Waza M,Adachi H,Katsuno M,Minamiyama M,Sang C,Tanaka F,Inukai A,Doyu M,Sobue G

MA3-414 was used in western blot to investigate the role of 17-AAG in curing spinal and bulbar muscular atrophy as well as degradation of polyglutamine-expanded mutant AR

Sat Oct 01 00:00:00 UTC 2005

Expression and localization of adipophilin and perilipin in human fetal membranes: association with lipid bodies and enzymes involved in prostaglandin synthesis.

The Journal of clinical endocrinology and metabolism

Meadows JW,Pitzer B,Brockman DE,Myatt L

MA3-414 was used in immunohistochemistry to study the expression of adipophilin and perilipin in human fetal membranes at preterm and term.

Fri Apr 01 00:00:00 UTC 2005

Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes.

Molecular and cellular biology

Stavreva DA,Müller WG,Hager GL,Smith CL,McNally JG

MA3-414 was used in immunocytochemistry to investigate the role of chaperones and proteasomes during glucocorticoid receptor exchange.

Thu Apr 01 00:00:00 UTC 2004

Microsomal prostaglandin E synthase-1 is overexpressed in inflammatory bowel disease. Evidence for involvement of the transcription factor Egr-1.

The Journal of biological chemistry

Subbaramaiah K,Yoshimatsu K,Scherl E,Das KM,Glazier KD,Golijanin D,Soslow RA,Tanabe T,Naraba H,Dannenberg AJ

MA3-414 was used in western blot to study the effect of Egr-1-mediated transcription activation on mPGES-1 expression in inflammatory bowel disease .

Fri Mar 26 00:00:00 UTC 2004

Progesterone receptor deficient in chromatin binding has an altered cellular state.

The Journal of biological chemistry

Botos J,Xian W,Smith DF,Smith CL

MA3-414 was used in immunoprecipitation to analyze the altered cellular processing by comparing the composition and distribution of tPR and sPR complexes

Fri Apr 09 00:00:00 UTC 2004

FK506 requires stimulation of the extracellular signal-regulated kinase 1/2 and the steroid receptor chaperone protein p23 for neurite elongation.

Neuro-Signals

Gold BG,Zhong YP

MA3-414 was used in immunocytochemistry to study the role of the ERK and steroid receptor chaperone protein p23 in the neurite elongation activity of FK506

Tue Jul 06 00:00:00 UTC 2004

The Journal of biological chemistry

Felts SJ,Owen BA,Nguyen P,Trepel J,Donner DB,Toft DO

MA3-414 was used in immunoprecipitation to investigate the functional properties HSP90-related protein TRAP1 in mitochondria

Fri Feb 04 00:00:00 UTC 2000

Active participation of Hsp90 in the biogenesis of the trimeric reovirus cell attachment protein sigma1.

The Journal of biological chemistry

Gilmore R,Coffey MC,Lee PW

MA3-414 was used in immunoprecipitation to demonstrate that chaperones play an important role in the regulation of general protein folding and of steroid receptor activation

Fri Jun 12 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: Co chaperone p23; cPGES; cytosolic prostaglandin E synthase; Cytosolic prostaglandin E2 synthase; Hsp90 co chaperone; Hsp90 co-chaperone; p23 cochaperone; Progesterone receptor complex p23; Prostaglandin E synthase 3; prostaglandin E synthase 3 (cytosolic); prostaglandin-E synthase 3; prostaglandin-E synthase 3-like; ptges3 {ECO:0000250|UniProtKB:Q15185}; Sid 3177; telomerase binding protein, p23; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD

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Gene Aliases: 5730442A20Rik; cPGES; P23; Ptges; PTGES3; RGD1561913; Sid3177; TEBP

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UniProt ID: (Rabbit) G1TGF1, (Human) Q15185, (Rat) P83868, (Mouse) Q9R0Q7

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Entrez Gene ID: (Rabbit) 100345490, (Rabbit) 100351480, (Human) 10728, (Rat) 362809, (Mouse) 56351

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Function(s)

telomerase activity protein binding prostaglandin-E synthase activity unfolded protein binding p53 binding enzyme binding Hsp90 protein binding isomerase activity

Process(es)

prostaglandin biosynthetic process glycogen biosynthetic process RNA-dependent DNA biosynthetic process prostaglandin metabolic process cell proliferation positive regulation of gene expression sensory perception of pain glucocorticoid receptor signaling pathway skin development lung saccule development negative regulation of cell death chaperone cofactor-dependent protein refolding telomere maintenance signal transduction cyclooxygenase pathway regulation of cellular response to heat lipid metabolic process fatty acid metabolic process fatty acid biosynthetic process DNA biosynthetic process

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p23 Monoclonal Antibody (JJ3)

p23 Antibody (MA3-414) in ICC/IF

Product Details

MA3-414

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

in situ PLA (PLA)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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