Invitrogen

p25 Recombinant Rabbit Monoclonal Antibody (1E6C1)

Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-51499

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:6,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1E6C1

Immunogen

A synthesized peptide derived from human CDK5R1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3094324

Target Information

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: CDK5 activator 1; Cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; cyclin-dependent kinase 5, regulatory subunit (p35) 1; cyclin-dependent kinase 5, regulatory subunit 1 (p35); neuronal CDK5 activator; p23; regulatory partner for CDK5 kinase; tau protein kinase II 23 kDa subunit; tau protein kinase II 23kDa subunit; TPKII regulatory subunit

View more View less

Gene Aliases: CDK5P35; CDK5R; CDK5R1; D11Bwg0379e; NCK5A; p23; p25; p35; p35nck5a

View more View less

UniProt ID: (Human) Q15078, (Rat) P61810, (Mouse) P61809

View more View less

Entrez Gene ID: (Human) 8851, (Rat) 116671, (Mouse) 12569

View more View less

Function(s)
protein kinase activity cyclin-dependent protein serine/threonine kinase activity calcium ion binding protein binding kinase activity cyclin-dependent protein kinase 5 activator activity protein kinase binding protein serine/threonine kinase activator activity cadherin binding ephrin receptor binding cytoskeletal protein binding
Process(es)
neuron migration protein phosphorylation neuron cell-cell adhesion G-protein coupled acetylcholine receptor signaling pathway axon guidance axonal fasciculation brain development peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cerebellum development superior olivary nucleus maturation hippocampus development cerebral cortex radially oriented cell migration layer formation in cerebral cortex neuron differentiation negative regulation of axon extension neuron projection development regulation of actin cytoskeleton organization serine phosphorylation of STAT3 protein ionotropic glutamate receptor signaling pathway positive regulation of neuron apoptotic process positive regulation of protein kinase activity negative regulation of transcription, DNA-templated ephrin receptor signaling pathway rhythmic process axon extension regulation of dendritic spine morphogenesis regulation of microtubule cytoskeleton organization positive regulation of cell cycle arrest positive regulation of protein serine/threonine kinase activity positive regulation of protein targeting to membrane regulation of cyclin-dependent protein serine/threonine kinase activity cell proliferation embryo development regulation of macroautophagy regulation of neuron differentiation regulation of signal transduction by p53 class mediator regulation of cell cycle
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support