NeoBiotechnologies

p27Kip1 (Mitotic Inhibitor/Suppressor Protein) Monoclonal Antibody (SPM348)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

1027-MSM1X-P0

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

2-4 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SPM348

Immunogen

Purified GST-p27 fusion protein of Mouse origin
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Wet ice

Target Information

p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor 1B (p27 Kip1); Cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; cyclin-dependent kinase inhibitor p27/Kip1; P27 CDKN1B; p27(KIP1); p27Kip1

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Gene Aliases: AA408329; AI843786; Cdki1b; CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1

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UniProt ID: (Human) P46527, (Mouse) P46414

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Entrez Gene ID: (Human) 1027, (Mouse) 12576, (Rat) 83571

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Function(s)
cyclin-dependent protein serine/threonine kinase inhibitor activity transforming growth factor beta receptor, cytoplasmic mediator activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process protein phosphatase binding Hsp70 protein binding protein complex binding chaperone binding protein kinase inhibitor activity kinase activity
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle response to hypoxia potassium ion transport activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell cycle arrest Notch signaling pathway sensory perception of sound positive regulation of cell proliferation negative regulation of cell proliferation response to glucose positive regulation of cell death negative regulation of cell growth positive regulation of microtubule polymerization response to estradiol negative regulation of kinase activity negative regulation of phosphorylation response to drug negative regulation of apoptotic process response to amino acid response to peptide hormone positive regulation of protein catabolic process negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of mitotic cell cycle response to cadmium ion autophagic cell death inner ear development negative regulation of cellular component movement negative regulation of epithelial cell proliferation involved in prostate gland development cellular response to antibiotic cellular response to lithium ion cellular response to organic cyclic compound mitotic cell cycle arrest negative regulation of vascular smooth muscle cell proliferation cell cycle cell death regulation of cell proliferation negative regulation of epithelial cell proliferation response to organic cyclic compound phosphorylation
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