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Zeta
p27kip1 Monoclonal Antibody (SX53G8)
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Product Details
Z2158MS
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
SX53G8
Immunogen
Purified glutathione S-transferase (GST)-p27Kip1 fusion protein
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
tris with NP-40, BSA
Contains
<0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A recommended positive control tissue for this product is B-cell lymphoma, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
p27Kip1, a candidate tumor suppressor gene, functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-β induced G1 arrest. Clone SX53G8 co-precipitates cdk4 in complex with p27Kip1 and is excellent for staining of formalin fixed tissues.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
Target Information
p27 Kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest. p27 Kip1 is an important regulator of cell cycle progression, a potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. p27 Kip1 acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. p27 Kip1 has a molecular weight of approximately 27kDa and the degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDN1B; Cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; P27 CDKN1B; p27(KIP1); p27Kip1
Gene Aliases: CDKN1B; CDKN4; KIP1; MEN1B; MEN4; p27; P27KIP1
UniProt ID: (Human) P46527
Entrez Gene ID: (Human) 1027
cyclin-dependent protein serine/threonine kinase inhibitor activity
transforming growth factor beta receptor, cytoplasmic mediator activity
protein binding
cysteine-type endopeptidase activator activity involved in apoptotic process
protein phosphatase binding
Hsp70 protein binding
protein complex binding
chaperone binding
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
response to hypoxia
potassium ion transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
cell cycle arrest
Notch signaling pathway
sensory perception of sound
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
positive regulation of cell death
negative regulation of cell growth
positive regulation of microtubule polymerization
response to estradiol
negative regulation of kinase activity
negative regulation of phosphorylation
response to drug
negative regulation of apoptotic process
response to amino acid
response to peptide hormone
positive regulation of protein catabolic process
negative regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of mitotic cell cycle
response to cadmium ion
autophagic cell death
inner ear development
negative regulation of cellular component movement
negative regulation of epithelial cell proliferation involved in prostate gland development
cellular response to antibiotic
cellular response to lithium ion
cellular response to organic cyclic compound
mitotic cell cycle arrest
negative regulation of vascular smooth muscle cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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