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Rockland
p29ING4 Polyclonal Antibody
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p29ING4 Antibody (600-101-284) in WB
Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates. This antibody was used at 1.0 µg/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Personnel Communication, Motoko Unoki, NCI, NIH.
Product Details
600-101-284
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.
Conjugate
Form
Liquid
Concentration
1.28 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Reactivity occurs against human p29 ING4 protein. The sequence is not present in isoforms 2 (see below). However, 100% homology is on record for this protein from human, dog, rat, and mouse. Cross reactivity with p29 ING4 protein from chimpanzee and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p29 ING4 proteins from other sources is not known.
Target Information
The ING4 gene belongs to the ING (Inhibitor of Growth) family of tumor suppressors. All five members of the ING protein family identified to date contain a highly conserved plant homeodomain (PHD) finger motif at the C-terminal end, which is found in transcription factors that modulate chromatin structure. ING proteins have been shown to form transcriptional complexes leading to activation of responsive genes that mediate a wide range of cellular functions, including growth arrest, DNA repair, gene transcription, apoptosis, and senescence. The candidate tumor suppressor ING4 has a role in brain tumor pathogenesis, and is involved in regulating tumor growth and angiogenesis. Expression of ING4 is reduced in gliomas compared to normal brain tissue, and ING4 reduction correlates with progression from lower to higher grades of tumors. ING4 physically interacts with the p65 (RelA) subunit of NF-kB and represses transcription of NF-kB responsive genes. Inactivating mutations in ING4 transcripts have been described in various human cancer cell lines, and deletion of the ING4 locus has been detected in 10-20% of human breast cancer cell lines and tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain my036 protein; candidate tumor suppressor p33 ING1 homolog; Inhibitor of growth protein 4; p29ING4
Gene Aliases: ING4; My036; p29ING4
UniProt ID: (Human) Q9UNL4
Entrez Gene ID: (Human) 51147
DNA replication
protein acetylation
apoptotic process
DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
cell cycle arrest
negative regulation of cell proliferation
chromatin modification
positive regulation of apoptotic process
histone H3 acetylation
histone H4-K5 acetylation
histone H4-K8 acetylation
histone H4-K12 acetylation
histone H4-K16 acetylation
negative regulation of transcription, DNA-templated
negative regulation of growth
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