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Invitrogen
p47phox Polyclonal Antibody
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FIGURE: 1 / 1
p47phox Antibody (PA5-95502) in WB
Western blot analysis of p47phox in Lane 1: human HeLa whole cell lysates, Lane 2: human blood lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse NIH3T3 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with p47phox polyclonal antibody (Product # PA5-95502) using a 0.5 µg/mL dilution, follo... View More
Product Details
PA5-95502
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NCF1 recombinant protein (Position: M1-D270).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807304
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human blood, rat thymus tissue, rat lung tissue, mouse NIH3T3 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
p47phox (NCF1) is required, along with NCF2 and membrane bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in the gene can result in granulomatous disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NADPH oxidase subunit (47 kD); NADPH oxidase subunit (47kDa); NCF-1; NCF-47K; neutrop; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; P47 phox; p47-phox; phagocyte oxidase 47 kDa; SH3 and PX domain-containing protein 1A
Gene Aliases:
Ncf-1; NCF-47K; NCF1; NCF1A; NOXO2; p47-phox; p47
UniProt ID: (Human) P14598, (Mouse) Q09014
Entrez Gene ID: (Human) 653361, (Mouse) 17969, (Rat) 114553
response to yeast
leukocyte mediated cytotoxicity
respiratory burst involved in defense response
protein targeting to membrane
leukotriene metabolic process
NADP catabolic process
apoptotic process
inflammatory response
cellular defense response
cell proliferation
response to bacterium
superoxide anion generation
defense response to bacterium
respiratory burst
negative regulation of smooth muscle contraction
hydrogen peroxide biosynthetic process
defense response to Gram-positive bacterium
defense response to fungus
oxidation-reduction process
neutrophil mediated killing of gram-positive bacterium
neutrophil mediated killing of fungus
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
superoxide metabolic process
positive regulation of catalytic activity
innate immune response
cell redox homeostasis
vascular endothelial growth factor receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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