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Invitrogen
p53 Recombinant Rabbit Monoclonal Antibody (014)
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FIGURE: 1 / 2
p53 Antibody (MA5-52458) in WB
Western blot analysis of p53 in A431 Whole Cell Lysate. Samples were incubated with p53 monoclonal antibody (Product # MA5-52458) using a dilution of 1:500 followed by Goat Anti-Rabbit IgG H&L (Dylight800) at a dilution of 1:10,000. Lane A: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 53 kDa; Observed band size: 53 kDa.
Product Details
MA5-52458
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
014
Immunogen
Recombinant Cynomolgus p53/TP53 protein (Met1-Asp393)
Conjugate
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3248935
Target Information
The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; OTTMUSP00000006194; Tumor suppressor p53; Tumour Protein p53
Gene Aliases: EGM_07376; P53; TP53
UniProt ID: (Cynomolgus monkey) P56423
Entrez Gene ID: (Cynomolgus monkey) 102135998
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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